Heintz N, Zernik M, Roeder R G
Cell. 1981 Jun;24(3):661-8. doi: 10.1016/0092-8674(81)90092-1.
We have isolated five clones containing human histone genes from a human genomic DNA library, using a cloned X. laevis histone H4 cDNA probe (pX1ch4). Each genomic clone has been mapped and the locations of the histone genes have been determined by blot hybridization and hybridization-selection of human histone mRNA. In contrast to the organization of previously characterized histone genes, the human histone genes are clustered in the genome but are not arranged into recognizable repeating units. The extreme lack of organization of the human histone genes may reflect the diminished requirement for rapid synthesis of large quantities of histone proteins during mammalian early development.
我们使用克隆的非洲爪蟾组蛋白H4 cDNA探针（pX1ch4），从一个人类基因组DNA文库中分离出了五个包含人类组蛋白基因的克隆。每个基因组克隆都已进行图谱绘制，并且通过人类组蛋白mRNA的印迹杂交和杂交筛选确定了组蛋白基因的位置。与先前表征的组蛋白基因的组织方式不同，人类组蛋白基因在基因组中是成簇的，但并未排列成可识别的重复单元。人类组蛋白基因极度缺乏组织性，这可能反映出在哺乳动物早期发育过程中，对快速合成大量组蛋白的需求减少。
