Monga D P
Infect Immun. 1981 Jun;32(3):975-8. doi: 10.1128/iai.32.3.975-978.1981.
Mice with either a stimulated or depressed reticuloendothelial system were used to study the role of macrophages in resistance to experimental Cryptococcus neoformans infection. Silica, administered intravenously to destroy macrophages, considerably decreased the phagocytic index of the reticuloendothelial system as determined by a carbon clearance test. Silica given 1 day before intravenous challenge with 5 X 10(3) (1 50% lethal dose) of C. neoformans markedly decreased the resistance of mice to cryptococcal infection. Mice given repeated doses of BCG to nonspecifically activate their macrophages could withstand a challenge of 100 50% lethal doses of C. neoformans. These results provide evidence that macrophages play an essential role in natural or nonspecific cell-mediated immunity to murine cryptococcosis.
使用网状内皮系统受到刺激或抑制的小鼠来研究巨噬细胞在抵抗新型隐球菌实验性感染中的作用。静脉注射二氧化硅以破坏巨噬细胞，通过碳清除试验测定，这显著降低了网状内皮系统的吞噬指数。在静脉注射5×10³（半数致死剂量的1.5倍）新型隐球菌前1天给予二氧化硅，显著降低了小鼠对隐球菌感染的抵抗力。给予小鼠重复剂量的卡介苗以非特异性激活其巨噬细胞，它们能够承受100个半数致死剂量的新型隐球菌的攻击。这些结果证明巨噬细胞在小鼠隐球菌病的天然或非特异性细胞介导免疫中起重要作用。
