Henze K, Chait A
Diabetologia. 1981 Jun;20(6):625-9. doi: 10.1007/BF00257431.
Methods for the measurement of the lysosomal enzymes acid cholesterol-ester hydrolase and N-acetyl-beta-glucosaminidase were adapted for use with freshly isolated circulating mononuclear cells. Activities of both these enzymes increased (to 259 and 147% of control values respectively) after 7 days of insulin therapy in diabetic subjects. Low density lipoprotein degradation by freshly isolated mononuclear cells increased simultaneously by 67% (p less than 0.05). The findings in this pilot study suggest [1] that circulating mononuclear cells can be used to evaluate the effect of metabolic changes in vivo on lysosomal enzymes and low density lipoprotein metabolism, and [2] that insulin can stimulate cellular lysosomal enzyme activity.
用于测量溶酶体酶酸性胆固醇酯水解酶和N-乙酰-β-葡萄糖胺酶的方法被调整用于新鲜分离的循环单核细胞。在糖尿病患者接受胰岛素治疗7天后，这两种酶的活性均增加（分别增至对照值的259%和147%）。新鲜分离的单核细胞对低密度脂蛋白的降解同时增加了67%（p<0.05）。这项初步研究的结果表明：[1]循环单核细胞可用于评估体内代谢变化对溶酶体酶和低密度脂蛋白代谢的影响；[2]胰岛素可刺激细胞溶酶体酶活性。
