Liburd E M, Pazderka V, Russell A S, Dossetor J B
Immunology. 1976 Nov;31(5):767-72.
Lymphocytes from thirty-six normal individuals of both sexes with ages ranging from 4-89 years were tested for their cytotoxic activity against short term cluters of fibroblasts from nine foetal umbilical cords and nine adult skin samples. Wide variability in the amount of fibroblast lysis was observed. Adult male lymphocytes caused significantly higher lytic activity than lymphocytes of adult females against both types of targets. Cell-mediated target cell reduction was not confined to allogeneic target cells. There was also killing of autochthonous cultured fibroblasts. Some individuals' sera inhibited the fibroblast lytic activity of their own lymphocytes. These cell-mediated reactions are directed against unknown antigenic specifications, probably not histocompatibility antigens, and give evidence of the problem of how to measure normal controls in the microcytotoxicity test in allogeneic human testing. Both autochthonous and allogeneic target cell lysis were also observed in SLE patients.
对36名年龄在4至89岁之间的正常男女的淋巴细胞进行了测试，检测其对来自9条胎儿脐带和成体皮肤样本的短期成纤维细胞集落的细胞毒性活性。观察到成纤维细胞裂解量存在很大差异。成年男性淋巴细胞对两种类型的靶细胞所产生的裂解活性明显高于成年女性淋巴细胞。细胞介导的靶细胞减少并不局限于同种异体靶细胞。自体培养的成纤维细胞也会被杀伤。一些个体的血清抑制了其自身淋巴细胞的成纤维细胞裂解活性。这些细胞介导的反应针对的是未知的抗原特性，可能不是组织相容性抗原，这也证明了在同种异体人体检测的微细胞毒性试验中如何测量正常对照这一问题。在系统性红斑狼疮患者中也观察到了自体和同种异体靶细胞的裂解。
