通过C型病毒转导来自不同物种的同源转化基因产生BALB-MuSV和Ha-MuSC。
Generation of BALB-MuSV and Ha-MuSC by type C virus transduction of homologous transforming genes from different species.
作者信息
Andersen P R, Devare S G, Tronick S R, Ellis R W, Aaronson S A, Scolnick E M
出版信息
Cell. 1981 Oct;26(1 Pt 1):129-34. doi: 10.1016/0092-8674(81)90041-6.
The nature of the cell-derived (bas) sequences of BALB-MuSV, a spontaneous mouse sarcoma virus isolate, was determined. Molecularly cloned bas sequences demonstrated no detectable homology with the onc genes of other mouse transforming viruses, but exhibited a high degree of sequence homology with the ras gene of the rat-derived Harvey murine sarcoma virus (Ha-MuSV) genome. The Ha-MuSV cell-derived sequence (ras) shared a colinear 750 bp region of homology with bas. Moreover, BALB-MuSV transformation was associated with the expression of high levels of a 21,000 dalton protein, immunologically related to the ras gene products, p21. Thus bas and ras represent retroviral transforming gene homologs that were independently transduced by mouse type C viruses from the genomes of different species.
确定了一种自发分离的小鼠肉瘤病毒BALB-MuSV的细胞衍生（bas）序列的性质。分子克隆的bas序列与其他小鼠转化病毒的癌基因没有可检测到的同源性，但与大鼠源哈维小鼠肉瘤病毒（Ha-MuSV）基因组的ras基因表现出高度的序列同源性。Ha-MuSV细胞衍生序列（ras）与bas共享一个750 bp的共线同源区域。此外，BALB-MuSV转化与高水平表达一种21,000道尔顿的蛋白质有关，该蛋白质在免疫上与ras基因产物p21相关。因此，bas和ras代表逆转录病毒转化基因同源物，它们由小鼠C型病毒从不同物种的基因组中独立转导而来。
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