酶联免疫吸附测定（ELISA）——一种用于检测和区分针对密切相关正痘病毒抗体的简单检测方法。
ELISA--a simple test for detecting and differentiating antibodies to closely related orthopoxviruses.
作者信息
Marennikova S S, Malceva N N, Habahpaseva N A
出版信息
Bull World Health Organ. 1981;59(3):365-9.
Abstract
In this paper, the results are reported of experiments to investigate the optimum conditions for determining, and differentiating between, the antibodies against closely related poxviruses using the ELISA technique. This assay was found to be considerably more sensitive than the virus neutralization, haemagglutination inhibition, passive haemagglutination, and indirect fluorescent antibody tests for detecting antibodies, but has not yet been compared with radioimmunoassay. The use of ELISA made it possible to differentiate between antibodies to vaccinia, whitepox, and monkeypox viruses.
摘要
本文报道了利用酶联免疫吸附测定（ELISA）技术研究确定和区分针对密切相关痘病毒抗体的最佳条件的实验结果。发现该检测方法在检测抗体方面比病毒中和试验、血凝抑制试验、被动血凝试验和间接荧光抗体试验灵敏得多，但尚未与放射免疫测定法进行比较。ELISA的应用使得区分抗痘苗病毒抗体、抗白痘病毒抗体和抗猴痘病毒抗体成为可能。
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ELISA--a simple test for detecting and differentiating antibodies to closely related orthopoxviruses.酶联免疫吸附测定（ELISA）——一种用于检测和区分针对密切相关正痘病毒抗体的简单检测方法。
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本文引用的文献
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Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.酶联免疫吸附测定（ELISA）。免疫球蛋白G的定量测定。
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Immunofluorescence test for persistent poxvirus antibodies.持续性痘病毒抗体的免疫荧光检测。
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Three specific antigens produced in vaccinia, variola, and monkeypox infections.牛痘、天花和猴痘感染中产生的三种特定抗原。
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The detection of viruses by enzyme-linked immunosorbent assay (ELISA).通过酶联免疫吸附测定（ELISA）检测病毒。
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Differentiation of variola, monkeypox, and vaccinia antisera by radioimmunoassay.通过放射免疫测定法鉴别天花、猴痘和牛痘抗血清。
Bull World Health Organ. 1977;55(5):613-23.
6
Enzyme-immunoassays for antibodies in measles, cytomegalovirus infections and after rubella vaccination.用于检测麻疹、巨细胞病毒感染及风疹疫苗接种后抗体的酶免疫测定法。
Br J Exp Pathol. 1976 Apr;57(2):243-7.
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Detection by radioimmunoassay of antibodies in human smallpox patients and vaccinees.通过放射免疫分析法检测天花患者和接种疫苗者体内的抗体。
J Clin Microbiol. 1975 Mar;1(3):311-7. doi: 10.1128/jcm.1.3.311-317.1975.
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