用于检测麻疹、巨细胞病毒感染及风疹疫苗接种后抗体的酶免疫测定法。
Enzyme-immunoassays for antibodies in measles, cytomegalovirus infections and after rubella vaccination.
作者信息
Voller A, Bidwell D E
出版信息
Br J Exp Pathol. 1976 Apr;57(2):243-7.
Abstract
Enzyme-immunoassays using an indirect method with alkaline phosphatase conjugated antiglobulins were satisfactory for detection of antibody to Measles and Cytomegalovirus. The antigen was passively adsorbed to polystyrene micro-harmagglutination plates for the assays. IgM antibody to Rubella was also detected by enzyme-immunoassay at 7 and 28 days after vaccination in a person who had negative Rubella serology before vaccination. IgG antibody was detected at those times in another patient who had positive Rubella serology prior to vaccination. The enzyme immunoassays appear to have the potential for routine laboratory use for virological diagnosis.
摘要
使用与碱性磷酸酶结合的抗球蛋白间接法的酶免疫测定法，对于检测麻疹抗体和巨细胞病毒抗体是令人满意的。抗原被被动吸附到聚苯乙烯微量血凝板上用于检测。在一名接种前风疹血清学呈阴性的人接种疫苗后第7天和第28天，也通过酶免疫测定法检测到风疹IgM抗体。在另一名接种前风疹血清学呈阳性的患者中，在这些时间检测到了IgG抗体。酶免疫测定法似乎有用于病毒学诊断的常规实验室应用潜力。
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