酶联免疫吸附测定法、固相抗体捕捉酶免疫测定法及电子显微镜用于轮状病毒感染常规诊断的比较
Comparison of ELISA, SPACE, and electron microscopy for the routine diagnosis of rotavirus infection.
作者信息
Wall R A, Mellars B J, Luton P, Boulding S
出版信息
J Clin Pathol. 1982 Jan;35(1):104-6. doi: 10.1136/jcp.35.1.104.
Abstract
Previous studies on the serological diagnosis of rotavirus infection have utilised locally produced antibodies. In this study we have compared two commercially produced assays, an ELISA (Rotazyme, Abbott) and a newly developed assay--solid phase aggregation of coupled erythrocytes (SPACE) (Wellcome Research Laboratories), with electron microscopy (EM). The SPACE test appeared less sensitive than EM. The ELISA was shown to be as sensitive as EM but more versatile. Our experience suggests that the ELISA could be successfully incorporated into the routine of any diagnostic laboratory.
摘要
以往关于轮状病毒感染血清学诊断的研究使用的是本地生产的抗体。在本研究中，我们将两种商业生产的检测方法，即酶联免疫吸附测定法（Rotazyme，雅培公司）和新开发的检测方法——偶联红细胞固相凝集试验（SPACE）（威康研究实验室），与电子显微镜检查（EM）进行了比较。结果显示，SPACE试验的敏感性低于EM。ELISA显示出与EM一样敏感，但用途更广。我们的经验表明，ELISA可以成功地纳入任何诊断实验室的常规检测流程。
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