用于检测轮状病毒的固相放射免疫测定法。
Solid-phase radioimmunoassay for the detection of rotavirus.
作者信息
Middleton P J, Holdaway M D, Petric M, Szymanski M T, Tam J S
出版信息
Infect Immun. 1977 May;16(2):439-44. doi: 10.1128/iai.16.2.439-444.1977.
Abstract
A solid-phase radioimmunoassay method has been developed for the detection of rotavirus in the form of a purified antigen and in stool. The parameters of the radioimmunoassay were examined and optimized to give high sensitivity and same-day results. Compared with electron microscopy, the assay is up to 10 times as sensitive for detection of the virus in stool and up to 128 times as sensitive for detection of a purified virus antigen. In a field study on stool specimens it was at least as efficient as electron microscopy.
摘要
已开发出一种固相放射免疫测定法，用于检测纯化抗原形式的轮状病毒以及粪便中的轮状病毒。对放射免疫测定的参数进行了检查和优化，以实现高灵敏度和当日出结果。与电子显微镜相比，该测定法检测粪便中病毒的灵敏度高达10倍，检测纯化病毒抗原的灵敏度高达128倍。在一项对粪便标本的现场研究中，其效率至少与电子显微镜相同。
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