在裸鼠体内维持生长的人类肿瘤中检测到一种与raf相关的及另外两种转化性DNA序列。
Detection of a raf-related and two other transforming DNA sequences in human tumors maintained in nude mice.
作者信息
Fukui M, Yamamoto T, Kawai S, Maruo K, Toyoshima K
出版信息
Proc Natl Acad Sci U S A. 1985 Sep;82(17):5954-8. doi: 10.1073/pnas.82.17.5954.
High molecular weight DNAs prepared from a variety of human tumors maintained in nude mice were assayed for their ability to transform NIH 3T3 cells. DNAs from 4 of 21 tumors tested induced transformed foci in cultures of NIH 3T3 cells. They were from a Ewing sarcoma line, a glioblastoma line, a leiomyosarcoma line, and a lung carcinoma line. Hybridization analyses of the NIH 3T3 transformant DNAs with a human repetitive sequence as probe revealed that four distinct transforming DNA sequences were transferred to NIH 3T3 cells from the four tumor lines. The transforming DNA in a lung carcinoma line was a human homologue of the oncogene of Kirsten murine sarcoma virus (Ki-ras). On the other hand, the three other transforming DNAs showed no similarity to any known human transforming gene detected by the NIH 3T3 transformation assay. Further analyses with a series of cloned oncogenes as probes revealed that the transforming DNA in a glioblastoma line was a human homologue of the oncogene of 3611-murine sarcoma virus (raf). However, the two transforming DNAs in a Ewing sarcoma line and a leiomyosarcoma line had no sequence homology to any of the cloned oncogenes.
对从裸鼠体内维持生长的多种人类肿瘤中提取的高分子量DNA，检测其转化NIH 3T3细胞的能力。在检测的21种肿瘤中，有4种肿瘤的DNA在NIH 3T3细胞培养物中诱导产生了转化灶。它们分别来自一个尤因肉瘤细胞系、一个胶质母细胞瘤细胞系、一个平滑肌肉瘤细胞系和一个肺癌细胞系。用人类重复序列作为探针，对NIH 3T3转化细胞的DNA进行杂交分析，结果显示，4种不同的转化DNA序列从这4种肿瘤细胞系转移到了NIH 3T3细胞中。一个肺癌细胞系中的转化DNA是克尔斯滕鼠肉瘤病毒（Ki-ras）癌基因的人类同源物。另一方面，其他三种转化DNA与通过NIH 3T3转化试验检测到的任何已知人类转化基因均无相似性。用一系列克隆癌基因作为探针进行进一步分析，结果显示，一个胶质母细胞瘤细胞系中的转化DNA是3611-鼠肉瘤病毒（raf）癌基因的人类同源物。然而，一个尤因肉瘤细胞系和一个平滑肌肉瘤细胞系中的两种转化DNA与任何克隆癌基因均无序列同源性。
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