Hudault S, Raibaud P, Ducluzeau R, Bridonneau C
Ann Microbiol (Paris). 1982 May-Jun;133(3):443-59.
Bacterial strains isolated from the digestive tract of conventional mice produced a barrier effect against a strain of Clostridium perfringens type A (strain CP) in the gastrointestinal tract of gnotobiotic mice. This barrier effect was observed when mice, monoassociated with Escherichia coli K12, were inoculated with a mixed culture of fusiform-shaped clostridia. The collection of fusiforms (collection D) was obtained from a single colony chosen from among the 26 types studied. Collection D and E. coli K12 together exerted a barrier effect against all strains of C. perfringens type A tested. No effect was observed against a C. perfringens type C strain. The expression of the barrier effect depended on the order in which the strains were used to inoculate the mice. Thus strain CP was eliminated from the digestive tract when the mice had previously been associated with collection D and E. coli K12. If the mice were inoculated with strain CP first, however, the barrier effect was only partial. We have also been able to use two strains of clostridia (C1, C3) instead of E. coli K12 to produce a drastic barrier effect against strain CP. The order in which the strains were used to inoculate the mice did not, in this case, influence the production of a barrier effect. Three strains (Da, Db, Dc) isolated from collection D, produced only a partial barrier effect against strain CP in mice previously associated with E. coli K12. These results illustrate the difficulties encountered in determining the minimal number of bacterial strains involved in the production of a barrier effect.
从常规小鼠消化道分离出的细菌菌株，在无菌小鼠的胃肠道中对A型产气荚膜梭菌菌株（CP菌株）产生了屏障作用。当与大肠杆菌K12单联的小鼠接种梭状芽孢杆菌混合培养物时，观察到了这种屏障作用。梭状芽孢杆菌菌群（菌群D）取自所研究的26种类型中的单个菌落。菌群D和大肠杆菌K12共同对所有测试的A型产气荚膜梭菌菌株发挥了屏障作用。对C型产气荚膜梭菌菌株未观察到作用。屏障作用的表现取决于用于接种小鼠的菌株顺序。因此，当小鼠先前与菌群D和大肠杆菌K12联用时，CP菌株从消化道中被清除。然而，如果小鼠首先接种CP菌株，屏障作用则只是部分的。我们还能够使用两株梭状芽孢杆菌（C1、C3）代替大肠杆菌K而对CP菌株产生显著的屏障作用。在这种情况下，用于接种小鼠的菌株顺序并不影响屏障作用的产生。从菌群D中分离出的三株菌株（Da、Db、Dc），对先前与大肠杆菌K12联的小鼠中的CP菌株仅产生部分屏障作用。这些结果说明了在确定产生屏障作用所涉及的最小细菌菌株数量时遇到的困难。
