使DNA聚合酶I的5'→3'核酸外切酶缺陷型大肠杆菌突变体恢复活力。
Restoration of viability to an Escherichia coli mutant deficient in the 5'----3' exonuclease of DNA polymerase I.
作者信息
Boling M, Adler H, Masker W
出版信息
J Bacteriol. 1984 Nov;160(2):706-10. doi: 10.1128/jb.160.2.706-710.1984.
Abstract
An Escherichia coli polA (Ex) mutant that is usually inviable at restrictive temperatures (43 degrees C) was found to grow normally at 43 degrees C when incubated in the presence of a membrane-containing fraction prepared from E. coli. This membrane fraction causes anaerobic conditions that are necessary but not sufficient for restoration of viability since some component present in the membrane fraction is also required for colony formation at 43 degrees C. The accumulation of small DNA fragments typical of aerobic growth of the polA(Ex) mutant was also seen under anaerobic conditions. The polA(Ex) strain was also much more sensitive than the isogenic wild-type strain to hydrogen peroxide.
摘要
一种通常在限制温度（43摄氏度）下无法存活的大肠杆菌polA（Ex）突变体，当在含有从大肠杆菌制备的含膜组分的情况下孵育时，发现在43摄氏度下能正常生长。该膜组分可导致厌氧条件，这对于恢复活力是必要的，但不是充分的，因为膜组分中存在的某些成分对于在43摄氏度下形成菌落也是必需的。在厌氧条件下也观察到了polA（Ex）突变体需氧生长典型的小DNA片段的积累。与同基因野生型菌株相比，polA（Ex）菌株对过氧化氢也更为敏感。
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