States J C, Connor W, Wosnick M A, Aiken J M, Gedamu L, Dixon G H
Nucleic Acids Res. 1982 Aug 11;10(15):4551-63. doi: 10.1093/nar/10.15.4551.
We have isolated, using nick-translated cloned protamine cDNA's as probes, several genomic clones containing protamine gene sequences from a Charon 4A library of Eco R1 digested rainbow trout (Salmo gairdnerii) DNA. One clone was chosen for detailed study and the 2.5 kbp Bam HI-Eco R1 restriction fragment containing the gene was subcloned in the plasmid pBR322. A 920 bp Bg1 II - Bam HI restriction fragment contains a sequence coding for protamine component CII as well as regions 5' and 3' to the mRNA coding portion. Present in the region 5' to the mRNA coding sequence are the promoter associated signals "TATA" box and "CAAT" box. The 5' untranslated region of the mRNA whose length and sequence were not established from the cDNA clones (1) was determined by nuclease mapping and starts within a sequence similar to the "capping signal" found in other genes. The protamine gene for CII contains no introns, a situation common to most histone genes, but, unlike the histone genes does not occur close to other protamine genes in a "cluster".
我们以缺口平移法克隆的鱼精蛋白互补脱氧核糖核酸（cDNA）作为探针，从经Eco R1酶切的虹鳟鱼（Salmo gairdnerii）DNA的Charon 4A文库中分离出了几个包含鱼精蛋白基因序列的基因组克隆。选择了一个克隆进行详细研究，并将包含该基因的2.5千碱基对（kbp）Bam HI - Eco R1限制性片段亚克隆到质粒pBR322中。一个920碱基对（bp）的Bg1 II - Bam HI限制性片段包含编码鱼精蛋白CII成分的序列以及mRNA编码部分的5'和3'区域。在mRNA编码序列的5'区域存在启动子相关信号“TATA”框和“CAAT”框。mRNA的5'非翻译区的长度和序列无法从之前的cDNA克隆（1）中确定，通过核酸酶作图确定其起始于一个与其他基因中发现的“加帽信号”相似的序列内。CII的鱼精蛋白基因不含内含子，这是大多数组蛋白基因常见的情况，但与组蛋白基因不同的是，它并不以“簇”的形式靠近其他鱼精蛋白基因存在。
