Machinami R, Kikuchi F, Matsushita H
Virchows Arch A Pathol Anat Histol. 1982;397(1):109-20. doi: 10.1007/BF00430898.
A case of epithelioid sarcoma was studied by electron microscopy and by light and electron microscopic enzyme histochemistry comparing with several control soft tissues. In addition to previously reported ultrastructural features, such as abundant 10 nm cytoplasmic filaments, desmosome-like cell junctions and small cystic spaces surrounded by filopodia or microvilli of the tumor cells, we encountered 10 nm cytoplasmic filaments showing electron dense condensation with a concentrically oriented or whorled pattern and a finger-print-like arrangement and 5'-nucleotidase activity of tumor cell membrane. Among the control soft tissues, 5'-nucleotidase activity was found only in synovial and endothelial cells. Both tumor and synovial cells showed no activity of adenosine triphosphatase, while marked activity of the enzyme was found in endothelial cells. These results support the concept that epithelioid sarcoma is derived from mesenchymal cells undergoing differentiation toward synovial cells during neoplastic transformation.
通过电子显微镜以及光镜和电镜酶组织化学方法，对一例上皮样肉瘤进行了研究，并与几种对照软组织进行了比较。除了先前报道的超微结构特征，如丰富的10nm细胞质细丝、桥粒样细胞连接以及被肿瘤细胞的丝状伪足或微绒毛包围的小囊腔外，我们还发现了呈同心取向或涡状模式以及指纹样排列的、显示电子致密凝聚的10nm细胞质细丝，以及肿瘤细胞膜的5'-核苷酸酶活性。在对照软组织中，仅在滑膜细胞和内皮细胞中发现了5'-核苷酸酶活性。肿瘤细胞和滑膜细胞均未显示三磷酸腺苷酶活性，而在内皮细胞中发现了该酶的显著活性。这些结果支持了上皮样肉瘤起源于肿瘤转化过程中向滑膜细胞分化的间充质细胞这一概念。
