棒状噬菌体ω（tox+）基因组中编码白喉毒素的基因的完整核苷酸序列。
The complete nucleotide sequence of the gene coding for diphtheria toxin in the corynephage omega (tox+) genome.
作者信息
Ratti G, Rappuoli R, Giannini G
出版信息
Nucleic Acids Res. 1983 Oct 11;11(19):6589-95. doi: 10.1093/nar/11.19.6589.
Abstract
A segment of corynephage omega (tox+) DNA, containing the gene for diphtheria toxin (tox) was fragmented with restriction enzymes and the fragments cloned into M13 vectors for nucleotide sequence determination. A long open reading frame was shown to encode the tox gene by comparing the predicted amino acid sequence with that of peptides derived from the mature toxin molecule. Analysis of the nucleotide sequence shows RNA polymerase and ribosome binding signals preceding a GTG codon in the open reading frame: if this is the correct starting signal for translation, then a 25 amino acid signal peptide can be predicted for the toxin molecule.
摘要
一段含有白喉毒素（tox）基因的棒状噬菌体ω（tox+）DNA，用限制性内切酶进行切割，然后将片段克隆到M13载体中用于核苷酸序列测定。通过将预测的氨基酸序列与源自成熟毒素分子的肽段的氨基酸序列进行比较，发现一个长的开放阅读框编码tox基因。核苷酸序列分析表明，在开放阅读框中的一个GTG密码子之前存在RNA聚合酶和核糖体结合信号：如果这是正确的翻译起始信号，那么可以预测毒素分子有一个25个氨基酸的信号肽。
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1
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Nucleic Acids Res. 1981 Dec 21;9(24):6907-15. doi: 10.1093/nar/9.24.6907.
2
A possible novel interaction between the 3'-end of 18 S ribosomal RNA and the 5'-leader sequence of many eukaryotic messenger RNAs.18 S核糖体RNA的3'端与许多真核生物信使RNA的5'前导序列之间可能存在一种新型相互作用。
FEBS Lett. 1982 Oct 18;147(2):133-6. doi: 10.1016/0014-5793(82)81026-0.
3
Structure-activity relationships of the B fragment of diphtheria toxin: the lipid-binding domains.白喉毒素B片段的构效关系：脂质结合结构域
Toxicon. 1982;20(1):243-6. doi: 10.1016/0041-0101(82)90209-4.
4
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Nucleic Acids Res. 1982 May 11;10(9):2971-96. doi: 10.1093/nar/10.9.2971.
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Science. 1983 Apr 29;220(4596):515-7. doi: 10.1126/science.6403984.
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