Gelmann E P, Clanton D J, Jariwalla R J, Rosenthal L J
Proc Natl Acad Sci U S A. 1983 Aug;80(16):5107-11. doi: 10.1073/pnas.80.16.5107.
Specific DNA fragments from human cytomegalovirus (HCMV) strains Towne and AD169 exhibited homology to myc DNA sequences under hybridization conditions corresponding to a 22-28% base mismatch. In a specific subset of hybridizing HCMV fragments, the homology was restricted to the 5' half of viral v-myc and the 5' half of human c-myc. No hybridization was observed between HCMV fragments and the 3' v-myc and 3' human c-myc probes. In Towne DNA, myc homologous sequences mapped in four regions within the long unique segment (0.070-0.094, 0.134-0.156, 0.454-0.470, and 0.591-0.605 map unit) and one region in each of the short terminal repeats (0.832-0.847 and 0.984-1.0 map unit). In strain AD169, myc homology mapped in three regions within the long unique segment (0.123-0.147, 0.174-0.198, and 0.583-0.606 map unit) and one region in each of the short terminal repeats (0.833-0.863 and 0.976-1.0 map unit). By utilizing probes specific for the 5' and 3' portions of v-myc and human c-myc, we established that the regions of homology in a specific subset of HCMV restriction fragments corresponded to the 5' half of myc and were not due to MC29 viral helper sequences, flanking cellular sequences, or binding of probe to G.C-rich DNA.
在对应22%-28%碱基错配的杂交条件下，来自人巨细胞病毒（HCMV）毒株汤氏株（Towne）和AD169株的特定DNA片段与myc DNA序列表现出同源性。在杂交的HCMV片段的一个特定子集中，同源性局限于病毒v-myc的5'端一半和人c-myc的5'端一半。未观察到HCMV片段与3' v-myc和3'人c-myc探针之间的杂交。在汤氏株DNA中，myc同源序列定位于长独特片段内的四个区域（0.070 - 0.094、0.134 - 0.156、0.454 - 0.470和0.591 - 0.605图单位）以及每个短末端重复序列中的一个区域（0.832 - 0.847和0.984 - 1.0图单位）。在AD169株中，myc同源性定位于长独特片段内的三个区域（0.123 - 0.147、0.174 - 0.198和0.583 - 0.606图单位）以及每个短末端重复序列中的一个区域（0.833 - 0.863和0.976 - 1.0图单位）。通过使用针对v-myc和人c-myc的5'端和3'端部分的特异性探针，我们确定HCMV限制性片段的一个特定子集中的同源区域对应于myc的5'端一半，并非由于MC29病毒辅助序列、侧翼细胞序列或探针与富含G.C的DNA结合所致。
