Bodescot M, Chambraud B, Farrell P, Perricaudet M
EMBO J. 1984 Aug;3(8):1913-7. doi: 10.1002/j.1460-2075.1984.tb02067.x.
We have constructed a cDNA library from the cytoplasmic RNAs of Raji cells, a Burkitt's lymphoma cell line latently infected with Epstein-Barr virus. We report here the characterization of a cDNA representing a spliced RNA transcribed from the IR1-U2 region of the viral genome. The cDNA is 1007 bp long. The 5' region contains three tandem repeats of two exons, 66 and 132 bp, which are transcribed from the IR1 repeats. The 3' region is formed from four exons transcribed from U2. An open reading frame extends from the 5' end to position 784, and includes the repeats. This reading frame presumably corresponds to the carboxy-terminal 261 amino acids of a polypeptide containing several repeats of a 66 amino acid sequence. Since it would be encoded by the IR1-U2 region of the viral genome, the putative polypeptide might be involved in the process of growth-transformation of B-lymphocytes.
我们从Raji细胞的细胞质RNA构建了一个cDNA文库，Raji细胞是一种潜伏感染爱泼斯坦-巴尔病毒的伯基特淋巴瘤细胞系。我们在此报告一个cDNA的特征，该cDNA代表从病毒基因组的IR1-U2区域转录的剪接RNA。该cDNA长1007 bp。5'区域包含两个外显子的三个串联重复序列，分别为66 bp和132 bp，它们从IR1重复序列转录而来。3'区域由从U2转录的四个外显子形成。一个开放阅读框从5'端延伸至第784位，并包括这些重复序列。这个阅读框大概对应于一个含有66个氨基酸序列的几个重复序列的多肽的羧基末端261个氨基酸。由于它将由病毒基因组的IR1-U2区域编码，推测的多肽可能参与B淋巴细胞的生长转化过程。
