Skog S, Collins V P, Ivarsson B, Tribukait B
Acta Radiol Oncol. 1983;22(2):151-62. doi: 10.3109/02841868309134355.
Following irradiation with 5 Gy, Ehrlich ascites tumour cells growing in vivo were separated by means of elutriator centrifugation into G1, S-phase and G2 + M cells. The surface and internal morphology of the cells was analysed using scanning and transmission electron microscopy and compared with non-irradiated controls. Marked changes of the cell surface were found 5 to 72 h after irradiation, with the development of long straight microvilli mainly in S-phase and G2 cells and the appearance of cells without microvilli in G1. The latter were generally associated with intracellular degenerative changes. The appearance of these microvilli, and the consequent increase in cellular surface area was correlated to enlargement of the Golgi complex. Changes in the distribution of intracellular organelles were noted 5 h after irradiation in cells from all stages of the cell cycle. An increase of the ratio of polyribosomes to ribosomes was found in S-phase and G2 cells up to 72 h. The appearance of long microvilli was not related to a redistribution of cells in cell cycle and occurred below doses at which changes in cell membrane function are usually found.
用5 Gy射线照射后，通过淘析离心法将体内生长的艾氏腹水瘤细胞分离为G1期、S期和G2 + M期细胞。利用扫描电子显微镜和透射电子显微镜分析细胞的表面和内部形态，并与未照射的对照进行比较。照射后5至72小时发现细胞表面有明显变化，主要在S期和G2期细胞中出现长而直的微绒毛，而在G1期出现无微绒毛的细胞。后者通常伴有细胞内退行性变化。这些微绒毛的出现以及随之而来的细胞表面积增加与高尔基体复合体的增大相关。在细胞周期各阶段的细胞中，照射后5小时观察到细胞内细胞器分布的变化。在S期和G2期细胞中，直至72小时，多核糖体与核糖体的比例增加。长微绒毛的出现与细胞周期中细胞的重新分布无关，且发生在通常发现细胞膜功能变化的剂量以下。
