通过酶联免疫吸附测定法测定单纯疱疹病毒型特异性抗体。
Determination of herpes simplex virus type-specific antibodies by enzyme-linked immunosorbent assay.
作者信息
Coleman R M, Pereira L, Bailey P D, Dondero D, Wickliffe C, Nahmias A J
出版信息
J Clin Microbiol. 1983 Aug;18(2):287-91. doi: 10.1128/jcm.18.2.287-291.1983.
Abstract
We determined type-specific antibodies to herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) by an indirect enzyme-linked immunosorbent assay, using as antigens HSV-1 glycoprotein gC-1 and a HSV-2-specific polypeptide purified on affinity columns of monoclonal antibodies. All sera were initially screened for HSV antibodies by the enzyme-linked immunosorbent assay with a pool of Triton X-100-extracted antigens of HSV-1- and HSV-2-infected HEp-2 cells. The titer of HSV antibodies was predicted from a linear regression curve based on the absorbance of the initial 1:50 serum dilution. The sensitivity and specificity of the screening assay and of the assay for type-specific antibodies were established.
摘要
我们通过间接酶联免疫吸附测定法，使用单纯疱疹病毒1型（HSV-1）糖蛋白gC-1和在单克隆抗体亲和柱上纯化的HSV-2特异性多肽作为抗原，测定了针对HSV-1和HSV-2的型特异性抗体。所有血清最初通过酶联免疫吸附测定法，用从感染HSV-1和HSV-2的HEp-2细胞中提取的Triton X-100抗原池进行HSV抗体筛查。根据基于初始1:50血清稀释液吸光度的线性回归曲线预测HSV抗体滴度。确定了筛查测定法和型特异性抗体测定法的敏感性和特异性。
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