用于检测单纯疱疹病毒1型（HSV-1）和单纯疱疹病毒2型（HSV-2）抗体的具有高灵敏度和特异性的流式细胞术间接免疫荧光测定法。
Flow cytometric indirect immunofluorescence assay with high sensitivity and specificity for the detection of antibodies to HSV-1 and HSV-2.
作者信息
Caruso A, Tinti M, Peroni L, Cabibbo E, De Rango C, Manca N, Turano A
机构信息
Institute of Microbiology, University of Brescia, Italy.
出版信息
Eur J Epidemiol. 1993 Sep;9(5):547-52. doi: 10.1007/BF00209534.
Cells infected with HSV-1 or HSV-2 develop viral antigens which can be detected by immunofluorescence. We developed a flow cytometric indirect immunofluorescence assay to detect and quantitate antibodies to HSV-1 and HSV-2 in human sera. Results obtained by flow cytometry for detecting antibodies against HSV-1, when compared with results obtained by ELISA, showed an index of overall agreement of 100%. The correlation between the antibody titers obtained with each method was found to be highly significant. An index of overall agreement equal to 94.1% was observed between results obtained by flow cytometry and by immunofluorescence as concerns the discrimination of HSV-2 positive from negative samples. However, the correlation between antibody titers was found to be not statistically significant. The flow cytometric assay proved to be type-specific.
感染单纯疱疹病毒1型（HSV - 1）或单纯疱疹病毒2型（HSV - 2）的细胞会产生可通过免疫荧光检测的病毒抗原。我们开发了一种流式细胞术间接免疫荧光测定法，用于检测和定量人血清中针对HSV - 1和HSV - 2的抗体。与酶联免疫吸附测定法（ELISA）获得的结果相比，流式细胞术检测针对HSV - 1抗体的结果显示总体一致性指数为100%。发现每种方法获得的抗体滴度之间的相关性非常显著。就区分HSV - 2阳性和阴性样本而言，流式细胞术和免疫荧光获得的结果之间观察到总体一致性指数为94.1%。然而，发现抗体滴度之间的相关性无统计学意义。流式细胞术测定法被证明具有型特异性。
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