Iqbal M J, Wilkinson M L, Johnson P J, Williams R
Br J Cancer. 1983 Dec;48(6):791-6. doi: 10.1038/bjc.1983.268.
Sex steroid receptor proteins were studied in human normal liver and hepatocellular carcinoma (HCC). Oestrogen receptor (ER) was detected in nucleosol and cytosol of 4 normal adult and 5 malignant liver specimens and in the cytosol of 6 foetal liver samples. Levels were 27.6-500 fmol mg-1 soluble protein in normal adults (Kd 1.48 X 10(-8) -1.12 X 10(-10) mol 1(-1) ), 45-290 fmol mg-1 in malignant liver tissue (Kd 3.26 X 10(-9) -3.64 X 10(-10) mol 1(-1] and a mean of 93 fmol mg-1 in foetal tissue (Kd 1.55 X 10(-9) mol 1(-1]. Androgen receptors (AR) were found only in cytosol and nucleosol of HCC (23-370 fmol mg-1) and in cytosol from foetal liver (29 fmol mg-1) with Kd from 2.90 X 10(-9) to 3.734 X 10(-10) mol 1(-1]. AR was distinguished from sex hormone binding globulin, which was also present in all cytosol samples, by the former's ability to selectively bind to methyltrienolone and the latter's absence from nucleosol. These findings provide further support for suggestions that oestrogen-related hepatic functions in man may be mediated by receptors and raise the possibility that hepatocellular carcinoma may be androgen dependent.
对人类正常肝脏和肝细胞癌（HCC）中的性类固醇受体蛋白进行了研究。在4例正常成人和5例恶性肝脏标本的核溶质和胞溶质以及6例胎儿肝脏样本的胞溶质中检测到雌激素受体（ER）。正常成人的水平为27.6 - 500 fmol mg-1可溶性蛋白（解离常数Kd为1.48×10(-8) - 1.12×10(-10) mol 1(-1)），恶性肝脏组织中的水平为45 - 290 fmol mg-1（Kd为3.26×10(-9) - 3.64×10(-10) mol 1(-1)），胎儿组织中的平均水平为93 fmol mg-1（Kd为1.55×10(-9) mol 1(-1)）。雄激素受体（AR）仅在HCC的胞溶质和核溶质中发现（23 - 370 fmol mg-1）以及胎儿肝脏的胞溶质中发现（29 fmol mg-1），解离常数Kd为2.90×10(-9)至3.734×10(-10) mol 1(-1)。AR通过其选择性结合甲基三烯olone的能力与也存在于所有胞溶质样本中的性激素结合球蛋白区分开来，并且在核溶质中不存在后者。这些发现进一步支持了人类中与雌激素相关的肝脏功能可能由受体介导的观点，并增加了肝细胞癌可能依赖雄激素的可能性。
