Ruta M, Wolford R, Dhar R, Defeo-Jones D, Ellis R W, Scolnick E M
Mol Cell Biol. 1986 May;6(5):1706-10. doi: 10.1128/mcb.6.5.1706-1710.1986.
We present the nucleotide sequence of the coding region of the rat c-rasH-1 gene and a partial sequence analysis of the rat c-rasH-2 gene. By comparing these sequences with the Harvey murine sarcoma virus ras gene, we predict that the p21 protein encoded by the Harvey virus differs from the cellular c-rasH-1-encoded p21 at only two amino acids; those at positions 12 and 59. Alterations at each of these positions may play a role in activating the viral p21 protein. The c-rasH-2 gene is likely to be a nonfunctional pseudogene because it lacks introns, cannot be activated to transform NIH 3T3 cells, and differs in sequence from both c-rasH-1 and v-rasH at several base pair positions.
我们展示了大鼠c-rasH-1基因编码区的核苷酸序列以及大鼠c-rasH-2基因的部分序列分析结果。通过将这些序列与哈维鼠肉瘤病毒ras基因进行比较，我们预测哈维病毒编码的p21蛋白与细胞c-rasH-1编码的p21蛋白仅在两个氨基酸上存在差异；分别位于第12和59位。这些位置上的每一个改变都可能在激活病毒p21蛋白中发挥作用。c-rasH-2基因可能是一个无功能的假基因，因为它缺乏内含子，不能被激活来转化NIH 3T3细胞，并且在几个碱基对位置上与c-rasH-1和v-rasH的序列都不同。
