Taylor D E, Garner R S, Allan B J
Antimicrob Agents Chemother. 1983 Dec;24(6):930-5. doi: 10.1128/AAC.24.6.930.
Tetracycline resistance in strains of Campylobacter jejuni and Campylobacter coli was mediated by plasmids. Intra- and interspecies transfer was demonstrated within the genus Campylobacter. Buoyant densities of plasmid DNAs ranged from 1.691 to 1.694 g/cm3 (31 to 33% guanine plus cytosine). Restriction enzymes AccI, BclI, BglII, and PstI were found to be most useful for comparing the plasmids. The molecular weight of C. jejuni plasmid pMAK175 was 44.7 kilobases (29 X 10(6), and the other plasmids had similar sizes. Two plasmids from Belgian isolates of C. coli of human origin had very similar restriction enzyme profiles and are probably identical. Plasmids from human isolates of C. jejuni originating in Canada and the animal isolate of C. coli showed greater diversity. DNA homology among the campylobacter plasmids was assessed by probing the digests with a nick-translated campylobacter plasmid, pMAK175. All restriction fragments showed significant homology with pMAK175 probe DNA. No homology was noted between campylobacter plasmid DNA and plasmids specifying the four classes of tetracycline resistance determinants found in Enterobacteriaceae.
空肠弯曲菌和结肠弯曲菌菌株中的四环素抗性由质粒介导。在弯曲菌属内证明了种内和种间转移。质粒DNA的浮力密度范围为1.691至1.694 g/cm³（鸟嘌呤加胞嘧啶含量为31%至33%）。发现限制性内切酶AccI、BclI、BglII和PstI对比较质粒最有用。空肠弯曲菌质粒pMAK175的分子量为44.7千碱基（29×10⁶），其他质粒大小相似。来自比利时人源结肠弯曲菌分离株的两个质粒具有非常相似的限制性内切酶图谱，可能是相同的。来自加拿大的人源空肠弯曲菌分离株和结肠弯曲菌动物分离株的质粒表现出更大的多样性。通过用缺口平移的弯曲菌质粒pMAK175探测消化产物来评估弯曲菌质粒之间的DNA同源性。所有限制性片段与pMAK175探针DNA都显示出显著的同源性。在弯曲菌质粒DNA与指定在肠杆菌科中发现的四类四环素抗性决定簇的质粒之间未发现同源性。
