Gronenborn A M, Clore G M, Jones M B, Jiricny J
FEBS Lett. 1984 Jan 9;165(2):216-22. doi: 10.1016/0014-5793(84)80172-6.
A 500 MHz 1H-NMR study on a synthetic DNA pentadecamer comprising the specific target site of the cAMP receptor protein in the ara BAD operon is presented. Using pre-steady state NOE measurements, unambiguous assignments of all the imino proton resonances and associated adenine (H2) resonances are obtained. From the NOE data interbase pair interproton distances involving the imino and adenine (H2) protons are determined. It is shown that these distances are very similar to those expected for classical B DNA (RMS difference of 0.5 A), but are significantly different from those expected for classical A DNA (RMS difference of 1.1 A).
本文介绍了一项针对包含阿拉伯糖操纵子中cAMP受体蛋白特定靶位点的合成DNA十五聚体的500兆赫¹H-NMR研究。通过预稳态NOE测量，获得了所有亚氨基质子共振和相关腺嘌呤（H2）共振的明确归属。根据NOE数据确定了涉及亚氨基和腺嘌呤（H2）质子的碱基对间质子距离。结果表明，这些距离与经典B型DNA预期的距离非常相似（均方根差异为0.5埃），但与经典A型DNA预期的距离有显著差异（均方根差异为1.1埃）。
