Lee T H, Coligan J E, McLane M F, Sodroski J G, Popovic M, Wong-Staal F, Gallo R C, Haseltine W, Essex M
Proc Natl Acad Sci U S A. 1984 Dec;81(23):7579-83. doi: 10.1073/pnas.81.23.7579.
People exposed to type I human T-cell leukemia virus (HTLV-I) develop antibodies to an antigen at the surface of virus-infected cells, designated human T-cell leukemia virus membrane antigen (HTLV-MA). In an earlier study, we demonstrated that the major component of HTLV-MA is gp61, a glycoprotein encoded by the HTLV env gene. In the current study, we found that human antibodies that react with HTLV-MA on cells infected with HTLV-I react equally well with HTLV-MA on C3-44/MO, a target cell infected with type II HTLV. A glycoprotein with an approximate size of 67 kDa, gp67, was identified in C3-44/MO using immunoprecipitation and NaDodSO4/PAGE analysis. The positions of serine and cysteine residues were determined in the amino terminus of gp67 by radiolabel sequencing analysis. Comparison with the amino acid sequence deduced from the primary nucleotide sequence of HTLV-IIMO virus reveals that gp67 is also encoded, at least in part, by the env gene. The gp67 of HTLV-IIMO, like the env gene product of HTLV-ICR, gp61, is recognized both by antibodies from a HTLV-IIMO-infected patient with a variant form of hairy cell leukemia, and by antibodies from patients with HTLV-I-associated adult T-cell leukemia/lymphoma. These results indicate that, despite the divergence between HTLV-I and HTLV-II, the major env gene products of the two types of HTLV are conserved to the degree that they are serologically cross-reactive.
暴露于I型人类T细胞白血病病毒（HTLV-I）的人会产生针对病毒感染细胞表面一种抗原的抗体，该抗原被命名为人类T细胞白血病病毒膜抗原（HTLV-MA）。在早期研究中，我们证明HTLV-MA的主要成分是gp61，这是一种由HTLV env基因编码的糖蛋白。在当前研究中，我们发现与感染HTLV-I的细胞上的HTLV-MA发生反应的人类抗体，与感染II型HTLV的靶细胞C3-44/MO上的HTLV-MA反应同样良好。使用免疫沉淀和NaDodSO4/PAGE分析在C3-44/MO中鉴定出一种大小约为67 kDa的糖蛋白gp67。通过放射性标记测序分析确定了gp67氨基末端丝氨酸和半胱氨酸残基的位置。与从HTLV-IIMO病毒的初级核苷酸序列推导的氨基酸序列进行比较表明，gp67至少部分也是由env基因编码的。HTLV-IIMO的gp67，与HTLV-ICR的env基因产物gp61一样，既被一名患有变异型毛细胞白血病的HTLV-IIMO感染患者的抗体识别，也被HTLV-I相关成人T细胞白血病/淋巴瘤患者的抗体识别。这些结果表明，尽管HTLV-I和HTLV-II之间存在差异，但这两种类型HTLV的主要env基因产物在血清学上具有交叉反应性，保守程度较高。
