使用噬菌体P1作为Tn5插入诱变的载体。
Use of bacteriophage P1 as a vector for Tn5 insertion mutagenesis.
作者信息
Quinto M, Bender R A
出版信息
Appl Environ Microbiol. 1984 Feb;47(2):436-8. doi: 10.1128/aem.47.2.436-438.1984.
Abstract
Infection of a strain lysogenic for bacteriophage P1 CM with P1::Tn5 followed by simultaneous selection for the chloroamphenicol resistance associated with the resident prophage and the kanamycin resistance associated with Tn5 results in a large number of independent Tn5 insertion mutations. This superinfection-selection protocol is a fast, easy, and safe way to isolate null mutations in enteric bacteria without generating unwanted cryptic mutations elsewhere in the genome.
摘要
用P1::Tn5感染对噬菌体P1 CM呈溶原性的菌株，随后同时选择与驻留原噬菌体相关的氯霉素抗性和与Tn5相关的卡那霉素抗性，会产生大量独立的Tn5插入突变。这种超感染选择方案是一种快速、简便且安全的方法，可用于在肠道细菌中分离无效突变，而不会在基因组的其他位置产生不需要的隐蔽突变。
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