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用环化乙肝病毒（HBV）DNA转染未经筛选的细胞培养物中乙肝表面抗原的表达

Expression of hepatitis B surface antigen in unselected cell culture transfected with recircularized HBV DNA.

作者信息

Wang Y, Schäfer-Ridder M, Stratowa C, Wong T K, Hofschneider P H

出版信息

EMBO J. 1982;1(10):1213-6. doi: 10.1002/j.1460-2075.1982.tb00015.x.

DOI:10.1002/j.1460-2075.1982.tb00015.x
PMID:6327256
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC553191/
Abstract

Hepatitis B virus (HBV) DNA was isolated from the recombinant plasmid pA01 -HBV and recircularized . Immediately after introduction of this DNA into mouse fibroblasts (NIH 3T3) we observed increasing release of hepatitis B surface antigen (HBsAg) into the culture medium. Later production of HBsAg declined to a lower but constant level. No dominant selective marker and foreign promoter were necessary in this system, which therefore can be used for the study of regulation of HBsAg expression.

摘要

从重组质粒pA01 - HBV中分离出乙肝病毒（HBV）DNA并使其环化。将该DNA导入小鼠成纤维细胞（NIH 3T3）后，我们立即观察到乙肝表面抗原（HBsAg）向培养基中的释放量增加。随后，HBsAg的产量下降至较低但稳定的水平。该系统无需显性选择标记和外源启动子，因此可用于研究HBsAg表达的调控。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d95/553191/eac7b9e9e411/emboj00302-0069-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d95/553191/689bc5ac0353/emboj00302-0067-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d95/553191/84234d25f65a/emboj00302-0068-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d95/553191/eac7b9e9e411/emboj00302-0069-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d95/553191/689bc5ac0353/emboj00302-0067-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d95/553191/84234d25f65a/emboj00302-0068-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d95/553191/eac7b9e9e411/emboj00302-0069-a.jpg

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本文引用的文献

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