Jaffe D M, Terry R D, Spiro A J
J Neurol Sci. 1978 Feb;35(2-3):189-200. doi: 10.1016/0022-510x(78)90002-3.
A morphometric study of disuse atrophy was done on the rat anterior tibilalis muscle over a 13-day period after immobilization of the hind leg by pinning the knee and ankle joints. Cross-sectional areas of individual muscle fibers were measured using image analysis, a new and precise technique. Three types of muscle fibers, light, medium and dark, were defined using a modified myosin-ATPase reaction on cryostat-cut sections. Each of the 3 fiber types was found to show a degree and time course of atrophy unique to itself. At the 0.01 confidence level, the dark fibers (with strongest ATPase reaction) did not shrink significantly. Both the light (ATPase negative) and medium fiber did show significant atrophy at that level; the light shrinking more than the medium. Simple visualization of the light microscopic images was often found to be misleading. Thus, the need was documented for a precise technique for analysis of fiber size distribution.
通过固定大鼠膝关节和踝关节来使其后腿固定，在13天的时间里，对大鼠胫前肌进行了废用性萎缩的形态计量学研究。使用图像分析这一新型精确技术测量了单个肌纤维的横截面积。在冷冻切片上采用改良的肌球蛋白 - ATP酶反应，定义了三种类型的肌纤维，即浅色、中等色和深色。发现这三种纤维类型中的每一种都呈现出自身独特的萎缩程度和时间进程。在0.01的置信水平下，深色纤维（ATP酶反应最强）没有明显萎缩。浅色（ATP酶阴性）和中等色纤维在该水平下均出现了明显萎缩；浅色纤维萎缩程度大于中等色纤维。人们常常发现光镜图像的简单观察具有误导性。因此，证明了需要一种精确的技术来分析纤维大小分布。
