Norton J D, Connor J, Avery R J
Nucleic Acids Res. 1984 Apr 25;12(8):3445-60. doi: 10.1093/nar/12.8.3445.
We have determined the nucleotide sequence and mapped the transcriptional boundaries in the long terminal repeats (LTRs) and adjacent regions of a retrovirus transmissible virus-like 30S ( VL30 ) mouse genetic element. The 572 base pair LTRs contain transcriptional regulatory sequences and are bounded by short imperfect repeats, with a minus strand tRNAgly primer binding site and a purine rich plus strand primer site flanking each of their inner boundaries. The 3' end of each LTR consists of an extensive 80 base pair redundancy of tRNA primer site and inverted repeat sequences while 41 and 47 base pair imperfect tandem repeats are present between the 5' capping site and the putative polyadenylation signal. Comparison with other retrovirus-like LTR sequences suggests possible modes of recombination that could occur between VL30 and other genetic elements.
我们已经确定了一种逆转录病毒样可传播30S（VL30）小鼠遗传元件的长末端重复序列（LTR）及其相邻区域的核苷酸序列，并绘制了转录边界。572个碱基对的LTR包含转录调控序列，由短的不完全重复序列界定，其内侧边界两侧分别有一个负链tRNAgly引物结合位点和一个富含嘌呤的正链引物位点。每个LTR的3'端由tRNA引物位点和反向重复序列的80个碱基对的广泛冗余组成，而在5'帽位点和假定的聚腺苷酸化信号之间存在41和47个碱基对的不完全串联重复序列。与其他逆转录病毒样LTR序列的比较表明，VL30与其他遗传元件之间可能发生重组的模式。
