在阿昔洛韦存在的情况下，对单纯疱疹病毒感染细胞中合成的小DNA片段的鉴定。
Identification of small DNA fragments synthesized in herpes simplex virus-infected cells in the presence of acyclovir.
作者信息
McGuirt P V, Shaw J E, Elion G B, Furman P A
出版信息
Antimicrob Agents Chemother. 1984 Apr;25(4):507-9. doi: 10.1128/AAC.25.4.507.
Abstract
The effect of acyclovir on DNA synthesized in cells infected with herpes simplex virus type 1 was examined. DNA that was synthesized in infected cells in the presence of acyclovir during a short pulse with [3H]thymidine remained near the top of an alkaline sucrose gradient after centrifugation. The sedimentation characteristics of labeled DNA were not changed after chasing in isotope-free medium. The slowly sedimenting DNA was identified as viral in origin by hybridization to purified herpes simplex virus nucleocapsid DNA. When cells were infected with acyclovir-resistant virus containing mutations in the polymerase gene, the viral DNA synthesized in the presence of acyclovir was chased into high-molecular-weight DNA. These findings are consistent with chain termination of herpes simplex virus DNA in virus-infected cells.
摘要
研究了阿昔洛韦对感染1型单纯疱疹病毒的细胞中合成的DNA的影响。在短时间用[³H]胸腺嘧啶脉冲处理期间，在阿昔洛韦存在下于感染细胞中合成的DNA在离心后仍保留在碱性蔗糖梯度的顶部附近。在无同位素培养基中追踪后，标记DNA的沉降特性未改变。通过与纯化的单纯疱疹病毒核衣壳DNA杂交，将沉降缓慢的DNA鉴定为病毒来源。当细胞感染在聚合酶基因中含有突变的阿昔洛韦抗性病毒时，在阿昔洛韦存在下合成的病毒DNA被追踪为高分子量DNA。这些发现与病毒感染细胞中单纯疱疹病毒DNA的链终止一致。
相似文献
1
Identification of small DNA fragments synthesized in herpes simplex virus-infected cells in the presence of acyclovir.
Antimicrob Agents Chemother. 1984 Apr;25(4):507-9. doi: 10.1128/AAC.25.4.507.
2
Acyclovir inhibition of viral DNA chain elongation in herpes simplex virus-infected cells.
Am J Med. 1982 Jul 20;73(1A):67-71. doi: 10.1016/0002-9343(82)90066-3.
3
Improved DNA hybridization method for detection of acyclovir-resistant herpes simplex virus.
Antimicrob Agents Chemother. 1987 Oct;31(10):1465-9. doi: 10.1128/AAC.31.10.1465.
4
Effect of acyclovir on viral protein synthesis in cells infected with herpes simplex virus type 1.
Antimicrob Agents Chemother. 1983 Feb;23(2):332-4. doi: 10.1128/AAC.23.2.332.
5
Mode of antiviral action of penciclovir in MRC-5 cells infected with herpes simplex virus type 1 (HSV-1), HSV-2, and varicella-zoster virus.
Antimicrob Agents Chemother. 1992 Dec;36(12):2747-57. doi: 10.1128/AAC.36.12.2747.
6
Metabolic fate of [3H]1-beta-D-arabinofuranosyl-5-[(E)-2-bromovinyl]uracil in herpes simplex virus type 1-infected cells.
Microbiol Immunol. 1997;41(12):957-64. doi: 10.1111/j.1348-0421.1997.tb01955.x.
7
Metabolism and mode of action of (R)-9-(3,4-dihydroxybutyl)guanine in herpes simplex virus-infected vero cells.
J Biol Chem. 1986 Feb 15;261(5):2134-9.
8
Herpes simplex virus resistant to acyclovir. A study in a tertiary care center.
Ann Intern Med. 1990 Mar 15;112(6):416-22. doi: 10.7326/0003-4819-76-3-112-6-416.
9
Significance of resistance of herpes simplex virus to acyclovir.
J Am Acad Dermatol. 1988 Jan;18(1 Pt 2):190-5. doi: 10.1016/s0190-9622(88)70026-2.
10
Isolation and characterization of herpes simplex virus resistant to nucleoside analogs.
Oral Surg Oral Med Oral Pathol. 1991 Sep;72(3):296-9. doi: 10.1016/0030-4220(91)90218-2.
引用本文的文献
1
Study of Hydrolysis Kinetics and Synthesis of Single Isomer of Phosphoramidate ProTide-Acyclovir.
ACS Omega. 2024 Nov 1;9(45):45221-45231. doi: 10.1021/acsomega.4c06645. eCollection 2024 Nov 12.
2
RNA Polymerase II Promoter-Proximal Pausing and Release to Elongation Are Key Steps Regulating Herpes Simplex Virus 1 Transcription.
J Virol. 2020 Feb 14;94(5). doi: 10.1128/JVI.02035-19.
3
The enzymological basis for resistance of herpesvirus DNA polymerase mutants to acyclovir: relationship to the structure of alpha-like DNA polymerases.
Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):447-52. doi: 10.1073/pnas.96.2.447.
4
Herpes simplex virus type 1 prereplicative sites are a heterogeneous population: only a subset are likely to be precursors to replication compartments.
J Virol. 1997 Jun;71(6):4771-81. doi: 10.1128/JVI.71.6.4771-4781.1997.
5
Evaluation of the polymerase chain reaction for diagnosis of herpes simplex virus encephalitis.
J Clin Microbiol. 1993 Jan;31(1):146-8. doi: 10.1128/jcm.31.1.146-148.1993.
6
Inhibition of human cytomegalovirus replication by 9-(1,3-dihydroxy-2-propoxymethyl)guanine alone and in combination with human interferons.
Antimicrob Agents Chemother. 1984 Oct;26(4):441-5. doi: 10.1128/AAC.26.4.441.
7
Inhibition by ganciclovir of cell growth and DNA synthesis of cells biochemically transformed with herpesvirus genetic information.
Antimicrob Agents Chemother. 1987 Jun;31(6):844-9. doi: 10.1128/AAC.31.6.844.
8
Prolonged herpes simplex virus latency in vitro after treatment of infected cells with acyclovir and human leukocyte interferon.
Antimicrob Agents Chemother. 1986 Apr;29(4):589-93. doi: 10.1128/AAC.29.4.589.
9
Plaque autoradiography assay for the detection and quantitation of thymidine kinase-deficient and thymidine kinase-altered mutants of herpes simplex virus in clinical isolates.
Antimicrob Agents Chemother. 1985 Aug;28(2):181-7. doi: 10.1128/AAC.28.2.181.
10
Inhibition of cellular DNA polymerase alpha and human cytomegalovirus-induced DNA polymerase by the triphosphates of 9-(2-hydroxyethoxymethyl)guanine and 9-(1,3-dihydroxy-2-propoxymethyl)guanine.
J Virol. 1985 Mar;53(3):776-80. doi: 10.1128/JVI.53.3.776-780.1985.
本文引用的文献
1
Inhibition of purified human and herpes simplex virus-induced DNA polymerases by 9-(2-hydroxyethoxymethyl)guanine triphosphate. Effects on primer-template function.
J Biol Chem. 1981 Nov 25;256(22):11447-51.
2
Acyclovir inhibition of Epstein-Barr virus replication.
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5163-6. doi: 10.1073/pnas.77.9.5163.
3
Inhibition by acyclovir of cell growth and DNA synthesis of cells biochemically transformed with herpesvirus genetic information.
Virology. 1980 Apr 30;102(2):420-30. doi: 10.1016/0042-6822(80)90109-9.
4
Size, composition, and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2.
J Virol. 1971 Aug;8(2):125-32. doi: 10.1128/JVI.8.2.125-132.1971.
5
Inhibition of herpes simplex virus-induced DNA polymerase activity and viral DNA replication by 9-(2-hydroxyethoxymethyl)guanine and its triphosphate.
J Virol. 1979 Oct;32(1):72-7. doi: 10.1128/JVI.32.1.72-77.1979.
6
Thymidine kinase from herpes simplex virus phosphorylates the new antiviral compound, 9-(2-hydroxyethoxymethyl)guanine.
J Biol Chem. 1978 Dec 25;253(24):8721-7.
7
Selectivity of action of an antiherpetic agent, 9-(2-hydroxyethoxymethyl) guanine.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5716-20. doi: 10.1073/pnas.74.12.5716.
Zotero 插件