McGuirt P V, Shaw J E, Elion G B, Furman P A
Antimicrob Agents Chemother. 1984 Apr;25(4):507-9. doi: 10.1128/AAC.25.4.507.
The effect of acyclovir on DNA synthesized in cells infected with herpes simplex virus type 1 was examined. DNA that was synthesized in infected cells in the presence of acyclovir during a short pulse with [3H]thymidine remained near the top of an alkaline sucrose gradient after centrifugation. The sedimentation characteristics of labeled DNA were not changed after chasing in isotope-free medium. The slowly sedimenting DNA was identified as viral in origin by hybridization to purified herpes simplex virus nucleocapsid DNA. When cells were infected with acyclovir-resistant virus containing mutations in the polymerase gene, the viral DNA synthesized in the presence of acyclovir was chased into high-molecular-weight DNA. These findings are consistent with chain termination of herpes simplex virus DNA in virus-infected cells.
研究了阿昔洛韦对感染1型单纯疱疹病毒的细胞中合成的DNA的影响。在短时间用[³H]胸腺嘧啶脉冲处理期间，在阿昔洛韦存在下于感染细胞中合成的DNA在离心后仍保留在碱性蔗糖梯度的顶部附近。在无同位素培养基中追踪后，标记DNA的沉降特性未改变。通过与纯化的单纯疱疹病毒核衣壳DNA杂交，将沉降缓慢的DNA鉴定为病毒来源。当细胞感染在聚合酶基因中含有突变的阿昔洛韦抗性病毒时，在阿昔洛韦存在下合成的病毒DNA被追踪为高分子量DNA。这些发现与病毒感染细胞中单纯疱疹病毒DNA的链终止一致。
