Chad J E, Eckert R
Biophys J. 1984 May;45(5):993-9. doi: 10.1016/S0006-3495(84)84244-7.
Computer-assisted modeling of calcium influx through voltage-activated membrane channels predicted that buffer-limited elevation of cytoplasmic free calcium ion concentration occurs within microscopic hemispherical "domains" centered upon the active Ca channels. With increasing depolarization, the number of activated channels, and hence the number of Ca domains, should increase; the single-channel current should, however, decrease, thereby decreasing Ca2+ accumulation in each domain relative to the macroscopic current. Such voltage dependence of the microscopic distribution of Ca2+ may influence relations between total Ca2+ entry and Ca-dependent processes. Ca-mediated inactivation of Ca channels in Aplysia neurons exhibits behavior consistent with the calcium domain hypothesis.
通过电压激活膜通道的钙内流的计算机辅助建模预测，细胞质游离钙离子浓度的缓冲受限升高发生在以活性钙通道为中心的微观半球形“区域”内。随着去极化增加，激活通道的数量以及因此钙区域的数量应该增加；然而，单通道电流应该减小，从而相对于宏观电流减少每个区域中的Ca2+积累。Ca2+微观分布的这种电压依赖性可能会影响总Ca2+内流与钙依赖性过程之间的关系。海兔神经元中钙通道的钙介导失活表现出与钙区域假说一致的行为。
