培养基对鲁米诺依赖性化学发光产生及测量的影响
The effect of culture media on the production and measurement of luminol-dependent chemiluminescence.
作者信息
Hastings M J, Petricevic I, Williams A J, Cole P J, Easmon C S
出版信息
Br J Exp Pathol. 1982 Apr;63(2):147-53.
Abstract
The effect of various media constituents on the production and measurement of luminol-dependent chemiluminescence by neutrophils stimulated by opsonized zymosan was studied. Glucose, calcium ions and magnesium ions were found to be necessary for optimal chemiluminescence although high concentrations of glucose had a detrimental effect. For our luminol-dependent system, a pH of 8.5 give maximal readings. The use of simple balanced salt solutions gave higher chemiluminescence responses than did more complex media with added protein, amino acids, and vitamins. However, the latter were important in the maintenance of optimal cell function.
摘要
研究了各种培养基成分对经调理酵母聚糖刺激的中性粒细胞产生和测量鲁米诺依赖性化学发光的影响。发现葡萄糖、钙离子和镁离子对于最佳化学发光是必需的，尽管高浓度葡萄糖有不利影响。对于我们的鲁米诺依赖性系统，pH值为8.5时读数最高。使用简单的平衡盐溶液比添加了蛋白质、氨基酸和维生素的更复杂培养基产生更高的化学发光反应。然而，后者对于维持最佳细胞功能很重要。
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