Hall S M, Confer A W, Tabatabai L B, Deyoe B L
J Clin Microbiol. 1984 Dec;20(6):1023-7. doi: 10.1128/jcm.20.6.1023-1027.1984.
A quantitative fluorometric immunoassay (FIAX) was adapted for the detection of serum antibodies to Brucella abortus in cattle. Results are expressed in nanograms of immunoglobulin binding the antigen carrier. The FIAX was compared with the standard tube agglutination, Rivanol precipitation, and complement fixation tests, using 285 serum samples from vaccinated, challenged, or control cattle. Linear regression analysis indicated a significant correlation among all four serological tests; the FIAX test correlated best with the Rivanol test. Ninety sera were from vaccinated and nonvaccinated cattle that were challenged with virulent B. abortus 2308. The sensitivity and specificity of each serological test were determined based on culture results from these cattle. The FIAX was the most sensitive of the four serological tests, detecting 79.2% of the culture-positive animals. The FIAX was the least specific, with 15.4% of the culture-negative animals being classified as positive. Eighty-eight sera were from cattle vaccinated with strain 19 but not challenged. All four serological tests had a statistically significant ability to distinguish sera from control and vaccinates on the basis of mean titers. The mean titer of vaccinates was also significantly different from that of challenged animals. Advantages and disadvantages of the FIAX test for bovine brucellosis are discussed.
一种定量荧光免疫测定法（FIAX）被用于检测牛血清中针对流产布鲁氏菌的抗体。结果以与抗原载体结合的免疫球蛋白的纳克数表示。使用来自接种、攻毒或对照牛的285份血清样本，将FIAX与标准试管凝集试验、瑞氏沉淀试验和补体结合试验进行比较。线性回归分析表明，所有四种血清学试验之间存在显著相关性；FIAX试验与瑞氏试验的相关性最佳。90份血清来自接种和未接种牛，这些牛用强毒流产布鲁氏菌2308进行攻毒。根据这些牛的培养结果确定每种血清学试验的敏感性和特异性。FIAX是四种血清学试验中最敏感的，检测出79.2%的培养阳性动物。FIAX的特异性最低，15.4%的培养阴性动物被分类为阳性。88份血清来自接种19号菌株但未攻毒的牛。所有四种血清学试验在基于平均滴度区分对照牛和接种牛的血清方面具有统计学上的显著能力。接种牛的平均滴度也与攻毒动物的平均滴度有显著差异。讨论了FIAX试验用于牛布鲁氏菌病的优缺点。
