Roth M G, Srinivas R V, Compans R W
J Virol. 1983 Mar;45(3):1065-73. doi: 10.1128/JVI.45.3.1065-1073.1983.
We have investigated the maturation sites of avian and mammalian C-type retroviruses in polarized epithelial cells. Examination of thin sections of Madin Darby canine kidney cells infected with RD114 or avian reticuloendotheliosis virus revealed that these viruses mature from the basolateral membrane domains. Similar results were obtained with a continuous line of mouse mammary epithelial cells infected with Friend, Moloney, Rauscher, or Kirsten murine leukemia viruses, or Friend virus-related or Moloney virus-related mink cell focus-forming viruses. Immunofluorescence observations indicate that viral glycoproteins are inserted only at the basolateral membranes in these cells. Because of the availability of DNA and protein sequence data, and of molecularly cloned viruses, these virus systems offer advantages for molecular studies on directional transport of plasma membrane glycoproteins.
我们研究了禽源和哺乳类C型逆转录病毒在极化上皮细胞中的成熟位点。对感染RD114或禽网状内皮组织增生症病毒的马-达二氏犬肾细胞薄片进行检查发现，这些病毒从基底外侧膜结构域成熟。用感染了Friend、莫洛尼、劳舍尔或柯斯顿鼠白血病病毒，或Friend病毒相关或莫洛尼病毒相关的貂细胞集落形成病毒的连续小鼠乳腺上皮细胞系也得到了类似结果。免疫荧光观察表明，病毒糖蛋白仅插入这些细胞的基底外侧膜。由于有DNA和蛋白质序列数据以及分子克隆病毒，这些病毒系统为质膜糖蛋白定向运输的分子研究提供了优势。
