Mayrhofer G, Whately R J
Int Arch Allergy Appl Immunol. 1983;71(4):317-27. doi: 10.1159/000233414.
Intraepithelial lymphocytes have been studied in the small intestinal epithelium of normal and thymus-deficient rats. A method is described that allows recovery of purified intraepithelial lymphocytes with high yield and viability. Granular intraepithelial lymphocytes were present in normal numbers in jejunal sections from nude rats and from adult thymectomized, irradiated, bone marrow-reconstituted rats. These probably corresponded to the cells with prominent cytoplasmic granules seen in smears of isolated intraepithelial lymphocytes. Studies in athymic chimeras indicated that both granular and nongranular intraepithelial cells differentiate from bone marrow without requirement for thymus processing and that they are relatively more radio-resistant than classical lymphocytes. It is suggested that all or most intraepithelial lymphocytes form a single cell lineage in which the degree of cytoplasmic differentiation is controlled by either the thymus or T lymphocytes. Intraepithelial lymphocytes are not postthymic T cells and their relationship to mucosal mast cells and to natural killer cells is discussed.
已对正常和胸腺缺陷大鼠小肠上皮内淋巴细胞进行了研究。本文描述了一种能以高产率和高活力回收纯化上皮内淋巴细胞的方法。裸鼠以及成年胸腺切除、照射、骨髓重建大鼠空肠切片中的颗粒状上皮内淋巴细胞数量正常。这些细胞可能与分离的上皮内淋巴细胞涂片上可见的具有明显胞质颗粒的细胞相对应。对无胸腺嵌合体的研究表明，颗粒状和非颗粒状上皮内细胞均由骨髓分化而来，无需胸腺处理，且它们比经典淋巴细胞对辐射相对更具抗性。有人提出，所有或大多数上皮内淋巴细胞形成单一细胞谱系，其中胞质分化程度受胸腺或T淋巴细胞控制。上皮内淋巴细胞不是胸腺后T细胞，并讨论了它们与黏膜肥大细胞和自然杀伤细胞的关系。
