哺乳动物细胞内各部分的电生理图谱。
The electrophysiological mapping of compartments within a mammalian cell.
作者信息
Giulian D, Diacumakos E G
出版信息
J Cell Biol. 1977 Jan;72(1):86-103. doi: 10.1083/jcb.72.1.86.
Abstract
The electrical properties of structures within an intact cell were examined by impalement with micropipette electrodes. Mean potential differences (PDs) measured from interphase HeLa cells showed that internal membrane-bounded compartments such as the nucleus, Golgi region, and the mitochondria were more negative than the cytoplasm with respect to an external grounding electrode. The nuclear PDs, unlike Golgi and cytoplasmic PDs, shifted during interphase and reached a peak value shortly before mitosis. The positioning of micropipettes was confirmed by electron microscope examination of marker solutions that were microinjected into specific intracellular regions. The combination of methods described here offers a new approach for the study of physiological events within intact, living cells.
摘要
通过用微电极刺入来检测完整细胞内结构的电学性质。从间期HeLa细胞测得的平均电位差（PDs）表明，相对于外部接地电极，诸如细胞核、高尔基体区域和线粒体等内部膜结合区室比细胞质更负。与高尔基体和细胞质的PDs不同，核PDs在间期会发生变化，并在有丝分裂前不久达到峰值。通过对微注射到特定细胞内区域的标记溶液进行电子显微镜检查，证实了微电极的定位。这里描述的方法组合为研究完整活细胞内的生理事件提供了一种新途径。
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