King L C, Loud K, Tejada S B, Kohan M J, Lewtas J
Environ Mutagen. 1983;5(4):577-88. doi: 10.1002/em.2860050407.
The release and recovery of mutagenic activity and 1-nitropyrene from diesel particles phagocytized and cultured with lung macrophages were studied. The Ames Salmonella typhimurium plate incorporation assay was used to measure mutagenic activity. Quantitative analysis of 1-nitropyrene was performed with liquid chromatography/fluorescence analysis. The cytotoxicity and phagocytosis of diesel particles with and without fetal calf serum were evaluated to select exposure concentrations that resulted in minimal toxicity and maximal engulfment of particles by the macrophages. The diesel-particle exposure concentrations for the mutagenicity studies were 200 micrograms/ml in the absence of serum and 375 micrograms/ml in the presence of serum. Engulfment and incubation of diesel particles with lung macrophages resulted in the loss of considerable mutagenic activity (97-98%) and significantly less 1-nitropyrene (10-25%). These studies suggest that lung macrophages have the capability to metabolize mutagenic nitroaromatics found in diesel particles.
研究了用肺巨噬细胞吞噬和培养的柴油颗粒中诱变活性和1-硝基芘的释放与回收情况。采用鼠伤寒沙门氏菌平板掺入法测定诱变活性。用液相色谱/荧光分析法对1-硝基芘进行定量分析。评估了有无胎牛血清时柴油颗粒的细胞毒性和吞噬作用，以选择导致最低毒性和巨噬细胞对颗粒最大吞噬量的暴露浓度。用于致突变性研究的柴油颗粒暴露浓度在无血清时为200微克/毫升，有血清时为375微克/毫升。柴油颗粒与肺巨噬细胞的吞噬和孵育导致相当大的诱变活性丧失（97-98%），1-硝基芘显著减少（10-25%）。这些研究表明，肺巨噬细胞有能力代谢柴油颗粒中发现的诱变硝基芳烃。
