采用改良裂解直接平板法快速检测儿童菌血症
Rapid detection of bacteremia in children with a modified lysis direct plating method.
作者信息
Campos J M, Spainhour J R
出版信息
J Clin Microbiol. 1985 Oct;22(4):674-6. doi: 10.1128/jcm.22.4.674-676.1985.
Abstract
A modified lysis direct plating method was developed for culturing blood specimens from pediatric patients. The method, which used the commercially available Isolator 1.5 Microbial Tube, consisted of direct inoculation of lysed blood to agar media and into a tube containing modified eugonic broth. During a 4,478-specimen study, 195 of 324 (60.2%) clinically significant isolates were detected within 24 h of specimen arrival in the laboratory. This method offers a rapid means to obtain quantitative blood culture results from pediatric patients and is an alternative to conventional and radiometric broth culture procedures.
摘要
开发了一种改良的裂解直接接种法用于培养儿科患者的血液标本。该方法使用市售的Isolator 1.5微生物管，包括将裂解后的血液直接接种到琼脂培养基和含有改良增菌肉汤的试管中。在一项对4478份标本的研究中，324份（60.2%）具有临床意义的分离株中有195份在标本送达实验室后的24小时内被检测到。该方法提供了一种从儿科患者中快速获得定量血培养结果的手段，是传统和放射性肉汤培养程序的一种替代方法。
相似文献
1
Rapid detection of bacteremia in children with a modified lysis direct plating method.采用改良裂解直接平板法快速检测儿童菌血症
J Clin Microbiol. 1985 Oct;22(4):674-6. doi: 10.1128/jcm.22.4.674-676.1985.
2
Comparison of the Isolator 1.5 Microbial Tube with a conventional blood culture broth system for detection of bacteremia in children.
Diagn Microbiol Infect Dis. 1985 Mar;3(2):167-74. doi: 10.1016/0732-8893(85)90027-6.
3
Clinical evaluation of a lysis-centrifugation technique for the detection of septicemia.用于检测败血症的溶解离心技术的临床评估。
JAMA. 1983 Oct 28;250(16):2185-8.
4
Evaluation of a lysis direct plating method for pediatric blood cultures.小儿血培养裂解直接接种法的评估
J Clin Microbiol. 1985 Jun;21(6):955-8. doi: 10.1128/jcm.21.6.955-958.1985.
5
Clinical comparison of the Isolator 1.5 microbial tube and the BACTEC radiometric system for detection of bacteremia in children.用于检测儿童菌血症的Isolator 1.5微生物培养管与BACTEC放射测量系统的临床比较
J Clin Microbiol. 1984 May;19(5):634-8. doi: 10.1128/jcm.19.5.634-638.1984.
6
Detection of bloodstream infections in children.儿童血流感染的检测
Eur J Clin Microbiol Infect Dis. 1989 Sep;8(9):815-24. doi: 10.1007/BF02185854.
7
Influence of a blood culture inoculation technique on detection of bacteremia by the BACTEC system.血培养接种技术对BACTEC系统检测菌血症的影响。
J Clin Microbiol. 1982 Sep;16(3):590-2. doi: 10.1128/jcm.16.3.590-592.1982.
8
Comparison of the BACTEC MYCO/F Lytic bottle to the isolator tube, BACTEC Plus Aerobic F/bottle, and BACTEC Anaerobic Lytic/10 bottle and comparison of the BACTEC Plus Aerobic F/bottle to the Isolator tube for recovery of bacteria, mycobacteria, and fungi from blood.比较BACTEC MYCO/F溶解瓶与隔离管、BACTEC Plus需氧F/瓶以及BACTEC厌氧溶解/10瓶，以及比较BACTEC Plus需氧F/瓶与隔离管从血液中分离细菌、分枝杆菌和真菌的能力。
J Clin Microbiol. 2001 Dec;39(12):4380-6. doi: 10.1128/JCM.39.12.4380-4386.2001.
9
Comparison of two commercially available media for detection of bacteremia.两种市售用于检测菌血症的培养基的比较。
Appl Microbiol. 1971 Oct;22(4):604-7. doi: 10.1128/am.22.4.604-607.1971.
10
Comparative analysis of two blood culture systems (Isolator and a 12-tube system) by cumulative differences in detection power at different times during incubation.通过对两种血培养系统（隔离器系统和12管系统）在培养不同时间段的检测能力累积差异进行比较分析。
APMIS. 1988 May;96(5):455-63. doi: 10.1111/j.1699-0463.1988.tb05329.x.
引用本文的文献
1
Volume of blood submitted for culture from neonates.提交用于新生儿血培养的血量。
J Clin Microbiol. 1986 Sep;24(3):353-6. doi: 10.1128/jcm.24.3.353-356.1986.
2
Blood culture updated.血培养结果更新。
Indian J Pediatr. 1986 Jul-Aug;53(4):429-31. doi: 10.1007/BF02749520.
3
Detection of bloodstream infections in children.儿童血流感染的检测
Eur J Clin Microbiol Infect Dis. 1989 Sep;8(9):815-24. doi: 10.1007/BF02185854.
4
Bacteremia detected by lysis direct plating in a neonatal intensive care unit.在新生儿重症监护病房通过裂解直接平板培养法检测到的菌血症。
J Clin Microbiol. 1990 Jan;28(1):1-4. doi: 10.1128/jcm.28.1.1-4.1990.
5
Quantitative aspects of septicemia.败血症的定量研究
Clin Microbiol Rev. 1990 Jul;3(3):269-79. doi: 10.1128/CMR.3.3.269.
本文引用的文献
1
Microbiological and clinical evaluation of the isolator lysis-centrifugation blood culture tube.隔离器裂解离心血液培养管的微生物学和临床评估
J Clin Microbiol. 1983 May;17(5):864-9. doi: 10.1128/jcm.17.5.864-869.1983.
2
Sepsis with coagulase-negative staphylococci in critically ill newborns.危重新生儿凝固酶阴性葡萄球菌败血症
Am J Dis Child. 1983 May;137(5):461-3. doi: 10.1001/archpedi.1983.02140310043012.
3
Relationship between the magnitude of bacteremia in children and the clinical disease.儿童菌血症程度与临床疾病之间的关系。
Pediatrics. 1982 Jun;69(6):699-702.
4
Diagnosis of bacteremia in children by quantitative direct plating and a radiometric procedure.采用定量直接接种法和放射性测量法诊断儿童菌血症。
J Clin Microbiol. 1981 Mar;13(3):478-82. doi: 10.1128/jcm.13.3.478-482.1981.
5
Quantitative blood cultures in the evaluation of septicemia in children with Broviac catheters.应用定量血培养评估带Broviac导管儿童的败血症。
J Pediatr. 1984 Jan;104(1):29-33. doi: 10.1016/s0022-3476(84)80584-3.
6
Clinical correlations of serial quantitative blood cultures determined by lysis-centrifugation in patients with persistent septicemia.采用裂解离心法对持续性败血症患者进行系列定量血培养的临床相关性研究。
J Clin Microbiol. 1984 Jun;19(6):766-71. doi: 10.1128/jcm.19.6.766-771.1984.
7
Clinical comparison of the Isolator 1.5 microbial tube and the BACTEC radiometric system for detection of bacteremia in children.用于检测儿童菌血症的Isolator 1.5微生物培养管与BACTEC放射测量系统的临床比较
J Clin Microbiol. 1984 May;19(5):634-8. doi: 10.1128/jcm.19.5.634-638.1984.
8
Contamination of cultures processed with the isolator lysis-centrifugation blood culture tube.使用隔离器裂解离心血液培养管处理的培养物受到污染。
J Clin Microbiol. 1984 Feb;19(2):97-9. doi: 10.1128/jcm.19.2.97-99.1984.
9
Early detection of positive blood cultures by the acridine orange staining technique.通过吖啶橙染色技术早期检测血培养阳性情况。
J Clin Microbiol. 1983 Oct;18(4):830-3. doi: 10.1128/jcm.18.4.830-833.1983.
10
Comparative recovery of bacteria and yeasts from lysis-centrifugation and a conventional blood culture system.裂解离心法与传统血培养系统对细菌和酵母菌的回收率比较
J Clin Microbiol. 1983 Aug;18(2):300-4. doi: 10.1128/jcm.18.2.300-304.1983.
Zotero 插件