Barnea B S, Fogel A
Central Virology Laboratory, Ministry of Health, Chaim Sheba Medical Center, Tel-Hashomer, Israel.
Isr J Med Sci. 1987 Dec;23(12):1223-7.
The use of SAM for the detection of rubella-specific IgM was evaluated in the sera of 318 rubella convalescents, 79 vaccinees and 53 infants with congenital rubella syndrome (CRS). The method employed was based on the use of crude inactivated suspension of Staphylococcus aureus strain Cowan I in the hemagglutination inhibition (HI) test and 1-h incubation of antigen and antibody. Among convalescents, 99% were found positive when tested within 30 days, and 84.6% were positive within 90 days following onset of clinical symptoms. Infants with CRS were 50 to 55% positive at the age of 0 to 6 months and 23% at the age of 7 to 11 months. Overnight incubation of antigen and antibody increased the proportion of positive results, particularly among sera of rubella vaccinees. No false-positives were detected among 1,035 healthy controls; nor in 40 patients following cytomegalovirus or Epstein-Barr virus infection, nor in 22 sera containing rheumatoid factor (RF). Comparison of SAM with a commercial enzyme-linked immunosorbent assay (ELISA) kit based on antibody capture showed 84% correlation between the two methods. Discrepancies were observed mainly in low-IgM.antibody-containing sera.
在318名风疹康复者、79名接种疫苗者和53名先天性风疹综合征（CRS）婴儿的血清中评估了金黄色葡萄球菌A蛋白（SAM）检测风疹特异性IgM的应用。所采用的方法是基于在血凝抑制（HI）试验中使用金黄色葡萄球菌考恩I株的粗制灭活悬液，并将抗原和抗体孵育1小时。在康复者中，30天内检测时99%呈阳性，临床症状出现后90天内84.6%呈阳性。CRS婴儿在0至6个月龄时50%至55%呈阳性，7至11个月龄时23%呈阳性。抗原和抗体过夜孵育增加了阳性结果的比例，尤其是在风疹疫苗接种者的血清中。在1035名健康对照者中未检测到假阳性；在40名巨细胞病毒或EB病毒感染后的患者中以及在22份含有类风湿因子（RF）的血清中也未检测到假阳性。将SAM与基于抗体捕获的商业酶联免疫吸附测定（ELISA）试剂盒进行比较，两种方法之间的相关性为84%。差异主要在含低IgM抗体的血清中观察到。
