Conway T A, Landon J, Smith D S, Shaw E J
Ther Drug Monit. 1983;5(3):347-53. doi: 10.1097/00007691-198309000-00018.
A latex-agglutination-inhibition test for serum gentamicin, based on inhibition by gentamicin of antibody-induced agglutination of gentamicin-coated latex particles, was found to be sufficiently reliable for use in therapeutic monitoring and to correlate well with a variety of established immunoassays. The test (a nonseparation, nonisotopic immunoassay) is performed on cards and has a simple visual end point by inspection for the presence or absence of agglutination. Severely elevated bilirubin or lipid levels, or gross hemolysis (which may cause interference with other nonseparation immunoassays) had no effect on the card test. With raised rheumatoid factor or complement, the cards gave accurate recovery of added gentamicin at 5 and 10 mg/L but low recovery at 2 mg/L. Of 67 patients' sera, three from one individual caused nonspecific agglutination of the latex and could not be assayed. The card test can be recommended for laboratories handling infrequent or small numbers of samples and for those without access to instrumentation.
一种基于庆大霉素对抗体诱导的庆大霉素包被乳胶颗粒凝集的抑制作用的血清庆大霉素乳胶凝集抑制试验，被发现用于治疗监测足够可靠，并且与多种已建立的免疫测定法相关性良好。该试验（一种非分离、非同位素免疫测定法）在卡片上进行，通过检查有无凝集有一个简单的视觉终点。严重升高的胆红素或脂质水平，或严重溶血（这可能会干扰其他非分离免疫测定法）对卡片试验没有影响。类风湿因子或补体升高时，卡片在5和10mg/L时对添加的庆大霉素回收率准确，但在2mg/L时回收率较低。在67例患者血清中，来自同一个体的3份血清导致乳胶非特异性凝集，无法进行测定。对于处理样本不频繁或数量少的实验室以及无法使用仪器的实验室，推荐使用卡片试验。
