血清中庆大霉素的生物发光测定法与琼脂扩散测定法、乳胶凝集抑制卡片试验、酶免疫测定法及荧光免疫测定法的相关性。
Correlation of bioluminescent assay of gentamicin in serum with agar diffusion assay, latex agglutination inhibition card test, enzyme immunoassay, and fluorescence immunoassay.
作者信息
Nilsson L
出版信息
J Clin Microbiol. 1984 Sep;20(3):396-9. doi: 10.1128/jcm.20.3.396-399.1984.
An improved bioluminescent assay of gentamicin in serum, based on the dose-dependent effect of the agent on the accumulation of extracellular ATP in Escherichia coli LU 14 cultures, is presented. The accuracy of the bioluminescent assay of gentamicin, expressed as the mean coefficient of variation over the therapeutic range, was 2.8%. Corresponding figures for an agar diffusion assay, a latex agglutination inhibition card test, an enzyme immunoassay, and a fluorescence immunoassay were 6.4, 17.5, 4.2, and 9.4%, respectively. All of the methods correlated well (r = 0.926 to 0.976), with the possible exception of the card test (r = 0.777 to 0.841). The bioluminescent assay requires only 1 microliter of serum, which allows for capillary sampling, and results are available within 75 min.
本文介绍了一种改进的血清庆大霉素生物发光检测方法，该方法基于庆大霉素对大肠杆菌LU 14培养物中细胞外ATP积累的剂量依赖性效应。庆大霉素生物发光检测的准确性，以治疗范围内的平均变异系数表示，为2.8%。琼脂扩散试验、乳胶凝集抑制卡试验、酶免疫测定和荧光免疫测定的相应数字分别为6.4%、17.5%、4.2%和9.4%。所有方法相关性良好（r = 0.926至0.976），卡试验可能除外（r = 0.777至0.841）。生物发光检测仅需1微升血清，允许毛细管采样，75分钟内即可获得结果。
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