酶联荧光免疫分析法检测沙眼衣原体IgM抗体
Detection of IgM antibodies against Chlamydia trachomatis by enzyme linked fluorescence immunoassay.
作者信息
Numazaki K, Chiba S, Yamanaka T, Moroboshi T, Aoki K, Nakao T
出版信息
J Clin Pathol. 1985 Jul;38(7):733-9. doi: 10.1136/jcp.38.7.733.
Abstract
A simple, sensitive enzyme linked fluorescence immunoassay has been developed to detect IgM antibodies against Chlamydia trachomatis. Reticulate bodies and elementary bodies from C trachomatis L2/434 Bu strain were isolated and used as antigens in the assay. Of 113 serum samples obtained from infants with pneumonia, 27 (23.9%) had IgM antibodies to C trachomatis L2 reticulate bodies and nine (8.0%) had IgM antibodies to C trachomatis L2 elementary bodies (titre greater than or equal to 1/500). Specific IgM antibodies were not detected in 20 control serum samples obtained from healthy adults and children. The possible use of enzyme linked fluorescence assay to determine IgM antibodies in the serodiagnosis of C trachomatis infection is discussed.
摘要
已开发出一种简单、灵敏的酶联荧光免疫分析法来检测抗沙眼衣原体的IgM抗体。从沙眼衣原体L2/434 Bu菌株中分离出网状体和原体，并将其用作该分析中的抗原。在从肺炎婴儿中获得的113份血清样本中，27份（23.9%）有针对沙眼衣原体L2网状体的IgM抗体，9份（8.0%）有针对沙眼衣原体L2原体的IgM抗体（滴度大于或等于1/500）。在从健康成人和儿童中获得的20份对照血清样本中未检测到特异性IgM抗体。讨论了酶联荧光分析法在沙眼衣原体感染血清诊断中用于测定IgM抗体的可能性。
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本文引用的文献
1
Immunoassay using antigen-enzyme conjugates.使用抗原-酶偶联物的免疫测定法。
FEBS Lett. 1971 Jun 24;15(3):232-236. doi: 10.1016/0014-5793(71)80319-8.
2
Comparison of fluorescent and colorigenic substrates for enzyme immunoassays.酶免疫测定中荧光底物与显色底物的比较。
J Clin Microbiol. 1982 May;15(5):757-60. doi: 10.1128/jcm.15.5.757-760.1982.
3
Enzyme-linked immunosorbent assay for immunoglobulin G and M antibodies to Chlamydia trachomatis in human sera.人血清中沙眼衣原体免疫球蛋白G和M抗体的酶联免疫吸附测定
J Clin Microbiol. 1983 May;17(5):848-52. doi: 10.1128/jcm.17.5.848-852.1983.
4
Development and evaluation of an enzyme-linked immunosorbent assay (ELISA), using chlamydial group antigen, to detect antibodies, to Chlamydia trachomatis.利用衣原体属群抗原开发和评估一种酶联免疫吸附测定法（ELISA），以检测沙眼衣原体抗体。
J Clin Pathol. 1982 Oct;35(10):1122-8. doi: 10.1136/jcp.35.10.1122.
5
Automated measurement of immunogalactosidase reactions with a fluorogenic substrate by the aperture defined microvolume measurement method and its potential application to Schistosoma mansoni immunodiagnosis.
J Immunol Methods. 1980;36(3-4):269-83. doi: 10.1016/0022-1759(80)90132-5.
6
Serology of Chlamydia trachomatis in infants.婴儿沙眼衣原体血清学
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7
Pneumonia due to Chlamydia trachomatis in Japanese infants.日本婴儿沙眼衣原体肺炎
Tohoku J Exp Med. 1984 Aug;143(4):413-20. doi: 10.1620/tjem.143.413.
8
Prevalence of antibodies to Chlamydia trachomatis in Japanese persons determined by microimmunofluorescence using reticulate bodies as single antigen.采用网状体作为单一抗原，通过微量免疫荧光法测定日本人沙眼衣原体抗体的流行情况。
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9
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J Lab Clin Med. 1983 Dec;102(6):918-25.
10
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