大肠杆菌中浮力细胞密度与生长速率的独立性
Independence of buoyant cell density and growth rate in Escherichia coli.
作者信息
Kubitschek H E, Baldwin W W, Schroeter S J, Graetzer R
出版信息
J Bacteriol. 1984 Apr;158(1):296-9. doi: 10.1128/jb.158.1.296-299.1984.
Abstract
The relationship between growth rate and buoyant density was determined for cells from exponential-phase cultures of Escherichia coli B/r NC32 by equilibrium centrifugation in Percoll gradients at growth rates ranging from 0.15 to 2.3 doublings per h. The mean buoyant density did not change significantly with growth rate in any of three sets of experiments in which different gradient conditions were used. In addition, when cultures were allowed to enter the stationary phase of growth, mean cell volumes and buoyant densities usually remained unchanged for extended periods. These and earlier results support the existence of a highly regulated, discrete state of buoyant density during steady-state growth of E. coli and other cells that divide by equatorial fission.
摘要
通过在Percoll梯度中进行平衡离心，测定了大肠杆菌B/r NC32指数生长期培养物中细胞的生长速率与浮力密度之间的关系，生长速率范围为每小时0.15至2.3代。在使用不同梯度条件的三组实验中的任何一组中，平均浮力密度均未随生长速率发生显著变化。此外，当培养物进入生长稳定期时，平均细胞体积和浮力密度通常会在较长时间内保持不变。这些结果以及早期的结果支持了在大肠杆菌和其他通过赤道分裂进行分裂的细胞的稳态生长过程中，存在一种高度调控的、离散的浮力密度状态。
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