用于评估麻疹病毒免疫力的酶联免疫吸附测定
Enzyme-linked immunosorbent assay for evaluation of immunity to measles virus.
作者信息
Weigle K A, Murphy M D, Brunell P A
出版信息
J Clin Microbiol. 1984 Mar;19(3):376-9. doi: 10.1128/jcm.19.3.376-379.1984.
Abstract
An enzyme-linked immunosorbent assay for the determination of immunity to measles virus was developed and standardized; it was compared to the hemagglutination inhibition and plaque reduction neutralization methods for sensitivity and specificity. The conditions of the enzyme-linked immunosorbent assay were adjusted such that groups of susceptible and immune individuals were clearly separable on the basis of the reactivity of a single (1:100) dilution of their sera to viral and control antigens. The range of values corresponding to susceptibility and immunity was defined by using the distribution of values observed from testing sera obtained from susceptible and immune control groups. The enzyme-linked immunosorbent assay was then applied in a study of measles vaccinees and found to be more sensitive than the hemagglutination inhibition method and equal in sensitivity to the plaque reduction neutralization method. The three methods were equal in specificity. Thus, the measles virus enzyme-linked immunosorbent assay is a rapid, reproducible, sensitive, and specific method for screening for the presence of measles antibody.
摘要
开发并标准化了一种用于测定麻疹病毒免疫力的酶联免疫吸附测定法；将其与血凝抑制法和蚀斑减少中和法在敏感性和特异性方面进行了比较。对酶联免疫吸附测定的条件进行了调整，使得易感个体组和免疫个体组能够根据其血清单一（1:100）稀释液对病毒抗原和对照抗原的反应性清晰区分开来。通过使用从易感和免疫对照组获得的检测血清所观察到的值的分布，确定了对应于易感性和免疫力的值的范围。然后将酶联免疫吸附测定法应用于麻疹疫苗接种者的研究，发现其比血凝抑制法更敏感，且在敏感性上与蚀斑减少中和法相当。这三种方法在特异性方面相当。因此，麻疹病毒酶联免疫吸附测定法是一种用于筛查麻疹抗体存在情况的快速、可重复、敏感且特异的方法。
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