Kischer C W, Hendrix M J
Cell Tissue Res. 1983;231(1):29-37. doi: 10.1007/BF00215771.
Fibronectin (FN) distribution was compared among samples of normal human dermis, hypertrophic scar, keloid, and granulation tissues from deep injuries. Localization was established by use of fibronectin antibodies and the indirect immunofluorescence method. Fresh-frozen tissue was sectioned on a cryostat and examined by epifluorescence. Hypertrophic scar and keloid demonstrated heavy deposition of FN, which conformed to the nodular characteristics of the lesions. Intense localization occurred in granulation tissue over fibroblasts which were stellate and vesiculated, and over small blood vessels. FN-staining was weak in areas over fibroblasts which were more rounded and nonvesiculated. Staining for FN was also minimal over the collagen in normal dermis and the deeper, larger collagen fascicles in the lesions. Fibroblasts cultured from normal dermis, hypertrophic scar, and keloid for 5-6 weeks were intensely stained for FN. Extracellular matrix was heavily positive in cultures from the lesions compared with those from normal dermis.
比较了正常人真皮、增生性瘢痕、瘢痕疙瘩以及深部损伤肉芽组织样本中的纤连蛋白（FN）分布情况。通过使用纤连蛋白抗体和间接免疫荧光法确定其定位。将新鲜冷冻组织在低温恒温器上切片，并用落射荧光显微镜检查。增生性瘢痕和瘢痕疙瘩显示出FN的大量沉积，这与病变的结节特征相符。在星状且有泡状的成纤维细胞上方以及小血管上方的肉芽组织中出现强烈定位。在更圆润且无泡状的成纤维细胞上方区域，FN染色较弱。在正常真皮中的胶原以及病变中更深、更大的胶原束上，FN染色也极少。从正常人真皮、增生性瘢痕和瘢痕疙瘩中培养5 - 6周的成纤维细胞，FN染色强烈。与正常真皮来源的培养物相比，病变来源培养物中的细胞外基质呈强阳性。
