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镉硫蛋白在离体大鼠肝细胞中的诱导作用。

Induction of cadmium-thionein in isolated rat liver cells.

作者信息

Hidalgo H A, Koppa V, Bryan S E

出版信息

Biochem J. 1978 Feb 15;170(2):219-225. doi: 10.1042/bj1700219.

DOI:10.1042/bj1700219
PMID:637838
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1183887/
Abstract

The uptake of cadmium by isolated liver cells was linearly related to the cadmium concentration to which the cells were exposed in the medium. Cadmium-treated cells synthesized proteins de novo with the characteristics of cadmium-thionein induced in the liver of cadmium-treated animals. Thionein from liver cells incorporated cadmium and [35S]cysteine, had a Ve/Vo (Sephadex G-50) of 1.8-1.9, and was separated into two subfractions by DEAE-cellulose ion-exchange chromatography. Cycloheximide and actinomycin D when added after a cadmium exposure prevented the synthesis of thionein. However, addition of actinomycin D after synthesis had started only decreased the total amount of thionein synthesized. The concentration of cadmium to which the cells were exposed affected the amount of cadmium-thionein synthesized in 6h. The maximum response occurred when cells were exposed to 0.5 microgram of cadmium/ml; at higher metal concentrations the total amount of cadmium-thionein synthesized declined. The system described in the present paper can be used to study the mode of metal toxicity and the mechanism of cadmium-thionein synthesis.

摘要

分离的肝细胞对镉的摄取与培养基中细胞所接触的镉浓度呈线性关系。经镉处理的细胞重新合成具有在镉处理动物肝脏中诱导产生的镉硫蛋白特性的蛋白质。肝细胞中的硫蛋白结合了镉和[35S]半胱氨酸，其Ve/Vo（葡聚糖G - 50）为1.8 - 1.9，并通过DEAE - 纤维素离子交换色谱法分离为两个亚组分。镉暴露后添加放线菌酮和放线菌素D可阻止硫蛋白的合成。然而，在合成开始后添加放线菌素D只会减少硫蛋白的总合成量。细胞所接触的镉浓度影响6小时内合成的镉硫蛋白量。当细胞接触0.5微克镉/毫升时出现最大反应；在更高的金属浓度下，合成的镉硫蛋白总量下降。本文所述系统可用于研究金属毒性模式和镉硫蛋白合成机制。

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本文引用的文献

1
Metallothionein: a cadmium- and zinc-containing protein from equine renal cortex.金属硫蛋白：一种来自马肾皮质的含镉和锌的蛋白质。
J Biol Chem. 1960 Dec;235:3460-5.
2
Isolation and some properties of human metallothionein.人金属硫蛋白的分离及某些特性
Biochemistry. 1966 May;5(5):1768-77. doi: 10.1021/bi00869a046.
3
Binding of mercury in the rat kidney by metallothionein.金属硫蛋白对大鼠肾脏中汞的结合作用。
Toxicol Appl Pharmacol. 1970 May;16(3):754-63. doi: 10.1016/0041-008x(70)90081-5.
4
Isolation of cadmium-binding proteins.镉结合蛋白的分离
Experientia. 1971 Sep 15;27(9):1024-5. doi: 10.1007/BF02138857.
5
Separation of two forms of rabbit metallothionein by isoelectric focusing.通过等电聚焦分离兔金属硫蛋白的两种形式。
Biochem J. 1972 Feb;126(3):491-8. doi: 10.1042/bj1260491.
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J Cell Biol. 1969 Dec;43(3):506-20. doi: 10.1083/jcb.43.3.506.
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Mercury binding in the kidney and liver of rats repeatedly exposed to mercuric chloride: induction of metallothionein by mercury and cadmium.反复接触氯化汞的大鼠肾脏和肝脏中的汞结合：汞和镉对金属硫蛋白的诱导作用。
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