利用单向辐射溶血法解读针对减毒甲型流感病毒的抗体反应及保护水平
Interpretation of responses and protective levels of antibody against attenuated influenza A viruses using single radial haemolysis.
作者信息
Al-Khayatt R, Jennings R, Potter C W
出版信息
J Hyg (Lond). 1984 Oct;93(2):301-12. doi: 10.1017/s0022172400064834.
Abstract
Antibody determinations against H3N2 and H1N1 type A influenza viruses were carried out on paired sera obtained from volunteers taking part in influenza virus vaccine studies, using both the haemagglutination-inhibition (HI) and single radial haemolysis (SRH) test. Good correlation between the HI and SRH test was found for both H3N2 and H1N1 antibody and the zone area increases corresponding to significant SRH antibody rises determined for both virus strains. In both H3N2 and H1N1 vaccine studies, intranasal infection of the volunteers with live attenuated viruses was involved and by the measurement of HI and SRH antibodies prior to and following infection, levels of antibody equating with protection against the infecting viruses could be estimated. For the HI test the antibody titres associated with 50% protection were 42 for H1N1, and 44 for H3N2 viruses; for the SRH test, 50% protection was associated with zone areas of 20.0-25.0 mm2 for both H1N1 and H3N2 viruses.
摘要
利用血凝抑制（HI）试验和单向辐射溶血（SRH）试验，对参与流感病毒疫苗研究的志愿者的配对血清进行了抗H3N2和H1N1甲型流感病毒抗体测定。对于H3N2和H1N1抗体，发现HI试验和SRH试验之间具有良好的相关性，并且对应于两种病毒株确定的显著SRH抗体升高，区域面积增加。在H3N2和H1N1疫苗研究中，均涉及用减毒活病毒对志愿者进行鼻内感染，并且通过在感染前后测量HI和SRH抗体，可以估计与预防感染病毒等效的抗体水平。对于HI试验，与50%保护相关的抗体滴度，H1N1病毒为42，H3N2病毒为44；对于SRH试验，对于H1N1和H3N2病毒，50%保护与20.0 - 25.0平方毫米的区域面积相关。
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