通过单向辐射溶血、中和及血凝抑制试验对马血清中马流感病毒抗体进行比较测定。
Comparative measurement of equine influenza virus antibodies in horse sera by single radial hemolysis, neutralization, and hemagglutination inhibition tests.
作者信息
Yamagishi H, Nagamine T, Shimoda K, Ide S, Igarashi Y, Yoshioka I, Matumoto M
出版信息
J Clin Microbiol. 1982 Apr;15(4):660-2. doi: 10.1128/jcm.15.4.660-662.1982.
Abstract
Single radial hemolysis (SRH), neutralization (NT), and hemagglutination inhibition (HI) tests were carried out on sera from horses immunized against the Prague and Miami strains of equine influenza virus. The HI and NT tests demonstrated good sensitivity; the sensitivity of the SRH test was somewhat lower. The NT titers of individual sera were correlated very closely with the HI titers, although the NT titers were higher. SRH zone diameters of individual sera also showed significant correlation with the NT and NI titers. The SRH test appears to be suitable for large-scale serological surveys and offers the advantages of rapidity and simplicity.
摘要
对免疫了马流感病毒布拉格株和迈阿密株的马匹血清进行了单向辐射溶血（SRH）、中和（NT）及血凝抑制（HI）试验。HI和NT试验显示出良好的敏感性；SRH试验的敏感性稍低。尽管NT效价较高，但各血清的NT效价与HI效价密切相关。各血清的SRH区直径也与NT和NI效价显著相关。SRH试验似乎适用于大规模血清学调查，具有快速、简便的优点。
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