[Electrophoretic study of pepsinogen in the gastric mucosa of white rats normally and with an experimental gastric stress ulcer].

The authors carried out electrophoretic studies of pepsinogen, obtained from the stomach mucosa of white rats (10 normal animals) and with experimental stress ulcus of the stomach, induced by imobilization and cooling (20 animals). The stomach of the rats was pulled out under light ether narcosis and the stomach mucosa was removed. Pepsinogen was extracted with 0.01 normal solution of hydrochloric acid. The supernatant was examined on agar electrophoresis after centrifugation. Incubation was made with hemoglobin solution of 0.66%. There were 4--5 pepsinogen fractions with well manifested proteolytic enzyme activity and sharply manifested ranges in the control animals. There was a reduction of the number of pepsinogen fraction in the animals with experimental stress ulcus of the stomach, as in 17 animals there was only one fraction with weak electrophoretic mobility, but with well manifested activity and in 3 animals--2 pepsinogen fractions. He discusses some questions about the increased cell-membrane permeability, accompanied by an increased release of pepsinogen and its preliminary activation in the stomach secretion.