Revenant M C
Clin Chem. 1983 Apr;29(4):681-3.
A "sandwich" enzyme immunoassay for ferritin in human serum is described, in which horseradish peroxidase-labeled antibody and a very sensitive chromogen, 2,2'-azino-di(3-ethyl-benzthiazolin-6-sulfonate), are used. The solid phase is polypropylene test tubes treated with glutaraldehyde; immunologic reactions are developed during constant rotation of the tubes. The assay requires 20 microL of serum per assay and takes less than 3 h. The standard curve is linear between 0 and 400 micrograms of serum ferritin per liter. Analytical recoveries of various amounts of standard added to a serum sample ranged from 92 to 98.6%. The sensitivity is 2 micrograms/L, which translates to 0.4 ng per assay tube. Within-run CV ranges from 1.6 to 5.4% and between-run from 4.2 to 7.8% according to the ferritin concentration. Results by competitive radioimmunoassay correlated well (r = 0.98). No "hook effect" was observed for concentrations up to 13 mg/L. The reference interval is 40-240 micrograms/L for men, 8-100 micrograms/L for women.
本文描述了一种用于检测人血清中铁蛋白的“夹心”酶免疫测定法，该方法使用了辣根过氧化物酶标记的抗体和一种非常灵敏的显色剂2,2'-叠氮基-二(3-乙基-苯并噻唑啉-6-磺酸盐)。固相为经戊二醛处理的聚丙烯试管；免疫反应在试管不断旋转的过程中进行。每次测定需要20微升血清，耗时不到3小时。标准曲线在每升血清铁蛋白0至400微克之间呈线性。向血清样本中添加不同量标准品的分析回收率在92%至98.6%之间。灵敏度为2微克/升，即每个测定管0.4纳克。根据铁蛋白浓度，批内变异系数在1.6%至5.4%之间，批间变异系数在4.2%至7.8%之间。竞争性放射免疫测定法的结果相关性良好（r = 0.98）。对于浓度高达13毫克/升的情况，未观察到“钩状效应”。男性的参考区间为40 - 240微克/升，女性为8 - 100微克/升。
